I-Polysaccharide evela kwi-Chlorella (PFC), njenge-polysaccharide yendalo, itsale ingqalelo enkulu kwiingcali kwiminyaka yakutshanje ngenxa yeenzuzo zayo zetyhefu ephantsi, iziphumo ebezingalindelekanga eziphantsi, kunye neziphumo ze-wide-spectrum. Imisebenzi yayo ekunciphiseni ii-lipids zegazi, ukulwa nomhlaza, ukulwa nokuvuvukala, ukulwa neParkinson's, ukulwa nokwaluphala, njl. iye yaqinisekiswa kwangaphambili kwi-in vitro nakwi-in vivo. Nangona kunjalo, kusekho umsantsa kuphando malunga ne-PFC njenge-human immune modulator.

Iiseli zeDendritic (iiDC) zezona ziseli zinamandla kakhulu ezibonisa i-antigen emzimbeni womntu. Inani leeDC emzimbeni womntu lincinci kakhulu, kwaye imodeli yokungenisa i-cytokine exhaswa yi-in vitro, oko kukuthi iiDCs ze-human peripheral blood mononuclear cell-derived DCs (ii-moDCs), isetyenziswa kakhulu. Imodeli ye-DC ebangelwa yi-in vitro yaxelwa okokuqala ngo-1992, eyinkqubo yenkcubeko yendabuko yeeDCs. Ngokubanzi, ifuna ukulinywa iintsuku ezi-6-7. Iiseli ze-mouse bone marrow zinokulinywa nge-granulocyte macrophage colony-stimulating factor (GM-CSF) kunye ne-interleukin (IL) -4 ukuze kufunyanwe iiDCs ezingavuthwanga (iqela le-PBS). IiCytokines zongezwa njengezinto ezivuthwayo kwaye zikhuliswe iintsuku ezi-1-2 ukuze kufunyanwe iiDCs ezivuthwayo. Olunye uphando lubike ukuba iiseli ze-CD14+ zabantu ezicociweyo zakhuliswa nge-interferon – β (IFN – β) okanye i-IL-4 kangangeentsuku ezi-5, zaze zakhuliswa nge-tumor necrosis factor-a (TNF-a) kangangeentsuku ezi-2 ukuze kufunyanwe ii-DCs ezine-CD11c kunye ne-CD83 ephezulu, ezinamandla aqinileyo okukhuthaza ukwanda kweeseli ze-allogeneic CD4+T kunye neeseli ze-CD8+T. Ii-polysaccharides ezininzi ezivela kwimithombo yendalo zinomsebenzi ogqwesileyo wokulawula amasosha omzimba, njengee-polysaccharides ezivela kwiikhowa ze-shiitake, iikhowa ze-split gill, iikhowa ze-Yunzhi, kunye ne-Poria cocos, eziye zasetyenziswa kwizonyango. Zinokuphucula ngempumelelo ukusebenza komzimba, zonyuse amasosha omzimba, kwaye zisebenze njengonyango oluncedisayo kunyango lokulwa ne-tumor. Nangona kunjalo, zimbalwa iingxelo zophando malunga ne-PFC njenge-immune modulator yomntu. Ke ngoko, eli nqaku lenza uphando lokuqala malunga nendima kunye neendlela ezinxulumene ne-PFC ekukhuthazeni ukuvuthwa kwee-moDCs, ukuze kuvavanywe amandla e-PFC njenge-immune modulator yendalo.

Ngenxa yomlinganiselo ophantsi kakhulu wee-DCs kwizicubu zabantu kunye nokugcinwa okuphezulu phakathi kweentlobo zezilwanyana phakathi kwee-DCs zegundane kunye nee-DCs zabantu, ukuze kusonjululwe ubunzima bophando obubangelwa yimveliso ephantsi ye-DC, iimodeli zokungenisa ii-DCs kwi-vitro ezivela kwiiseli ze-mononuclear zegazi lomntu ezikwi-peripheral ziye zafundwa, ezinokufumana ii-DCs ezine-immunogenicity entle ngexesha elifutshane. Ke ngoko, olu phononongo lusebenzise indlela yendabuko yokungenisa ii-DCs zabantu kwi-vitro: ukukhulisa i-rhGM CSF kunye ne-rhIL-4 kwi-vitro, ukutshintsha i-medium yonke imihla, kunye nokufumana ii-DCs ezingavuthwanga ngosuku lwesi-5; Ngomhla wesi-6, iivolumu ezilinganayo ze-PBS, PFC, kunye ne-LPS zongezwa ngokweqela kwaye zakhuliswa iiyure ezingama-24 njengeprotokholi yenkcubeko yokungenisa ii-DCs ezivela kwiiseli ze-mononuclear zegazi lomntu ezikwi-peripheral.

Iipolysaccharides ezivela kwimveliso yendalo zinezibonelelo zokuba netyhefu ephantsi kunye nexabiso eliphantsi njengee-immunostimulants. Emva kovavanyo lokuqala, iqela lethu lophando lifumanise ukuba i-PFC iyonyusa kakhulu uphawu oluvuthiweyo lwe-CD83 kumphezulu weeseli ze-DC ze-peripheral blood mononuclear cell-derived in vitro. Iziphumo ze-Flow cytometry zibonise ukuba ukungenelela kwe-PFC kuxinzelelo lwe-10 μ g/mL kangangeeyure ezingama-24 kubangele ukubonakaliswa okuphezulu kwe-CD83 yomphawuli ovuthiweyo kumphezulu weeDC, nto leyo ebonisa ukuba iiDC zingene kwimeko yokuvuthwa. Ke ngoko, iqela lethu lophando lichonge isicwangciso sokungeniswa kunye nokungenelela kwe-in vitro. I-CD83 yi-biomarker ebalulekileyo evuthiweyo kumphezulu weeDC, ngelixa i-CD86 isebenza njengemolekyuli ebalulekileyo yokuvuselela kumphezulu weeDC, isebenza njengomqondiso wesibini wokusebenzisa iiseli ze-T. Ukubonakaliswa okuphuculweyo kwee-biomarkers ezimbini ze-CD83 kunye ne-CD86 kubonisa ukuba i-PFC ikhuthaza ukuvuthwa kwee-DCs ze-peripheral blood mononuclear cell-derived, nto leyo ebonisa ukuba i-PFC inokunyusa ngaxeshanye inqanaba lokukhupha ii-cytokines kumphezulu weeDCs. Ngoko ke, olu phononongo luvavanye amanqanaba ee-cytokines IL-6, TNF-a, kunye ne-IL-10 ezikhutshwa yi-DCs zisebenzisa i-ELISA. I-IL-10 inxulumene kakhulu nokunyamezelana kwamajoni omzimba kwee-DCs, kwaye ii-DCs ezinokunyamezelana kwamajoni omzimba zisetyenziswa kakhulu kunyango lwe-tumor, zibonelela ngeengcinga zonyango ezinokubakho zokunyamezelana kwamajoni omzimba ekufakelweni kwamalungu omzimba; Usapho lwe-1L-6 ludlala indima ebalulekileyo kwi-innate and adaptive immunity, i-hematopoiesis, kunye neziphumo zokulwa nokuvuvukala; Kukho izifundo ezibonisa ukuba i-IL-6 kunye ne-TGF β zibandakanyeka kunye ekuhlukaniseni iiseli ze-Th17; Xa umzimba uhlaselwa yintsholongwane, i-TNF-a eveliswa yi-DCs ekuphenduleni ukusebenza kwentsholongwane isebenza njenge-autocrine maturity factor ukukhuthaza ukuvuthwa kwe-DC. Ukuthintela i-TNF-a kuya kubeka ii-DCs kwinqanaba lokungavuthwa, kuzithintele ekusebenziseni ngokupheleleyo umsebenzi wazo wokubonakaliswa kwe-antigen. Idatha ye-ELISA kolu phononongo ibonise ukuba inqanaba lokukhupha i-IL-10 kwiqela le-PFC landa kakhulu xa kuthelekiswa namanye amaqela amabini, okubonisa ukuba i-PFC inyusa ukunyamezelana kwamajoni omzimba kwee-DCs; Ukwanda kwamanqanaba okukhupha i-IL-6 kunye ne-TNF-a kubonisa ukuba i-PFC inokuba nefuthe lokuphucula i-DC ukukhuthaza ukwahluka kweeseli ze-T.