Polysaccharide ikomoka kuri Chlorella (PFC), nk'umuti usanzwe, yakuruye ibitekerezo byinshi by'abahanga mu myaka ya vuba aha kubera ibyiza byayo byo kuba ifite uburozi buke, ingaruka mbi nke, n'ingaruka zagutse. Imikorere yayo mu kugabanya ibinure mu maraso, kurwanya ikibyimba, kurwanya ububyimbirwe, kurwanya Parkinson, kurwanya gusaza, nibindi byemejwe mbere na mbere mu bushakashatsi bwakozwe mu buryo bwa vitro na in vivo. Ariko, haracyari icyuho mu bushakashatsi bwakozwe kuri PFC nk'umuti urinda umubiri w'umuntu.

Uturemangingo twa dendritic (DCs) nitwo turemangingo twihariye cyane twigaragaza mu mubiri w'umuntu. Umubare wa DCs mu mubiri w'umuntu ni muto cyane, kandi icyitegererezo cya cytokine gikoreshwa muri vitro, ni ukuvuga DCs zikomoka ku maraso y'umuntu (moDCs) (abantu bakomoka kuri DCs), ni cyo gikunze gukoreshwa. Icyitegererezo cya DC cyakozwe muri vitro cyatangajwe bwa mbere mu 1992, ari nacyo gikoresho gakondo cyo kwita ku dukoko twa DCs. Muri rusange, bisaba ko duhingwa mu gihe cy'iminsi 6-7. Uturemangingo tw'umufu w'imbeba dushobora guterwa hakoreshejwe granulocyte macrophage colony-stimulating factor (GM-CSF) na interleukin (IL) -4 kugira ngo haboneke DCs zidakuze (PBS group). Cytokines zongerwaho nk'ibintu bikuze kandi bigahingwa mu gihe cy'iminsi 1-2 kugira ngo haboneke DCs zikuze. Ubundi bushakashatsi bwagaragaje ko uturemangingo twa CD14+ tw’abantu twatunganyijwe hifashishijwe interferon – β (IFN – β) cyangwa IL-4 mu gihe cy’iminsi 5, hanyuma tugaterwa tumor necrosis factor-a (TNF-a) mu gihe cy’iminsi 2 kugira ngo haboneke DC zifite CD11c na CD83 nyinshi, zifite ubushobozi bukomeye bwo guteza imbere ikwirakwira ry’uturemangingo twa CD4+T na CD8+T. Polysaccharides nyinshi zikomoka ku bintu karemano zifite imikorere myiza yo guhindura ubudahangarwa bw’umubiri, nka polysaccharides zikomoka ku bihumyo bya shiitake, ibihumyo bya split gill, ibihumyo bya Yunzhi, na Poria cocos, byakoreshejwe mu buvuzi. Bishobora kunoza neza imikorere y’ubudahangarwa bw’umubiri, kongera ubudahangarwa bw’umubiri, no gukora nk’uburyo bw’inyongera mu kuvura kanseri. Ariko, hari raporo nke z’ubushakashatsi kuri PFC nk’uburyo bwo guhindura ubudahangarwa bw’umubiri bw’abantu. Kubwibyo, iyi nkuru ikora ubushakashatsi bw’ibanze ku ruhare n’uburyo bifitanye isano na PFC mu guteza imbere gukura kwa moDC, kugira ngo harebwe ubushobozi bwa PFC nk’uburyo bwo guhindura ubudahangarwa bw’umubiri bw’umwimerere.

Bitewe n’uko umubare muto cyane wa DC mu ngingo z’umuntu ndetse no kubungabungwa kw’ubwoko bwinshi hagati ya DC z’imbeba na DC z’umuntu, kugira ngo hakemurwe ibibazo by’ubushakashatsi biterwa no kuba DC ari nke, hakozwe ubushakashatsi ku miterere ya DC zikomoka ku turemangingo tw’amaraso y’abantu two mu maraso y’umuntu, zishobora kubona DC zifite ubushobozi bwo kwirinda indwara mu gihe gito. Kubwibyo, ubu bushakashatsi bwakoresheje uburyo gakondo bwo gutera DC z’umuntu muri vitro: guhuza rhGM CSF na rhIL-4 muri vitro, guhindura uburyo buri munsi, no kubona DC zitarakura ku munsi wa 5; Ku munsi wa 6, hongewemo ingano zingana za PBS, PFC, na LPS hakurikijwe ibyiciro no guterwa mu masaha 24 nk'uburyo bwo gutera DC zikomoka ku turemangingo tw’amaraso y’abantu two mu maraso y’umuntu.

Polysaccharides zikomoka ku bicuruzwa karemano zifite ibyiza byo kuba uburozi buke kandi igiciro gito nk'imiti itera imbaraga. Nyuma y'igerageza ry'ibanze, itsinda ryacu ry'ubushakashatsi ryasanze PFC yongera cyane ikimenyetso cy'ubukure cya CD83 ku buso bw'uturemangingo twa DC tw'amaraso y'umuntu dukomoka ku ngirabuzimafatizo z'amaraso y'umuntu dukomoka ku ngirabuzimafatizo z'amaraso. Ibisubizo bya cytometry y'umuvuduko w'amaraso byagaragaje ko PFC yakoreshejwe ku gipimo cya 10 μ g / mL mu gihe cy'amasaha 24 yatumye ikimenyetso cy'ubukure cya CD83 kigaragara cyane ku buso bwa DC, bigaragaza ko DC zinjiye mu mimerere yo gukura. Kubwibyo, itsinda ryacu ry'ubushakashatsi ryagennye gahunda yo gutangira no gukora in vitro. CD83 ni biomarker y'ingenzi ikuze ku buso bwa DC, mu gihe CD86 ikora nk'ikintu cy'ingenzi gitera imbaraga ku buso bwa DC, ikora nk'ikimenyetso cya kabiri cyo gukora uturemangingo twa T. Iterambere ry'iterambere ry'uturemangingo tubiri twa CD83 na CD86 bigaragaza ko PFC itera imbere gukura kwa DCs z'amaraso y'umuntu zikomoka ku ngirabuzimafatizo z'amaraso y'umuntu, bigaragaza ko PFC ishobora kongera icyarimwe urugero rw'isohoka rya cytokines ku buso bwa DCs. Kubwibyo, ubu bushakashatsi bwasuzumye urugero rwa cytokines IL-6, TNF-a, na IL-10 zitangwa na DCs hakoreshejwe ELISA. IL-10 ifitanye isano rya hafi n’ubudahangarwa bw’umubiri bwa DCs, kandi DCs zifite ubudahangarwa bw’umubiri zikunze gukoreshwa mu kuvura ikibyimba, zigatanga ibitekerezo by’ubuvuzi bwo kwihanganira ubudahangarwa bw’umubiri mu gutera ingingo; Umuryango wa 1L-6 ugira uruhare runini mu budahangarwa bw’umubiri n’ubudahangarwa, hematopoiesis, n’ingaruka zo kurwanya ububyimbirwe; Hari ubushakashatsi bwerekana ko IL-6 na TGF β bifatanya mu gutandukanya uturemangingo twa Th17; Iyo umubiri wibasiwe na virusi, TNF-a ikorwa na DCs mu gusubiza virusi ikora nk'ikintu cyo gukura cya autocrine kugira ngo gitere DCs gukura. Kubuza TNF-a bizashyira DCs mu cyiciro cyo gukura, bikayibuza gukoresha neza imikorere yayo yo kwerekana antigen. Amakuru ya ELISA muri ubu bushakashatsi yerekanye ko urugero rwo gusohora IL-10 mu itsinda rya PFC rwiyongereye cyane ugereranije n’andi matsinda abiri, bigaragaza ko PFC yongera ubudahangarwa bw’umubiri bwa DCs; Ukwiyongera kw'ibipimo bya IL-6 na TNF-a bigaragaza ko PFC ishobora kugira ingaruka zo kongera DC kugira ngo irusheho gutandukanya selile T.