Ko te polysaccharide mai i te Chlorella (PFC), he polysaccharide tūturu, kua kukume i te aro nui o ngā tohunga i ngā tau tata nei nā te mea he iti te paitini, he iti ngā pānga taha, me ngā pānga whānui. Ko ōna mahi ki te whakaheke i ngā ngako toto, te ārai i te pukupuku, te ārai i te mumura, te ārai i te mate a Parkinson, te ārai i te koroheketanga, me ētahi atu kua whakamanahia i mua i ngā whakamātautau in vitro me in vivo. Heoi, kei te takoto tonu he āputa i roto i te rangahau mō te PFC hei kaiwhakatikatika ārai mate tangata.

Ko ngā pūtau dendritic (DC) ngā pūtau whakaatu antigen motuhake tino kaha i roto i te tinana tangata. He tino iti te maha o ngā DC i roto i te tinana tangata, ā, he tauira whakaoho in vitro e whakahaerehia ana e te cytokine, arā, ko ngā DC mononuclear toto tangata (moDC), te mea e whakamahia whānuitia ana. I te tau 1992 i pūrongohia tuatahitia te tauira DC whakaoho in vitro, koinei te pūnaha whakatipu tuku iho mō ngā DC. I te nuinga o te wā, me whakatipu mō ngā rā 6-7. Ka taea te whakatipu i ngā pūtau wheua kiore me te granulocyte macrophage colony-stimulating factor (GM-CSF) me te interleukin (IL) -4 hei whiwhi DC kāore anō kia pakari (rōpū PBS). Ka tāpirihia ngā cytokine hei whakaoho pakeke, ā, ka whakatipuria mō ngā rā 1-2 hei whiwhi DC pakeke. I roto i tētahi atu rangahau, i kōrerohia ko ngā pūtau CD14+ tangata kua purea i whakatōngia ki te interferon – β (IFN – β) me te IL-4 rānei mō ngā rā e 5, kātahi ka whakatōngia ki te tumor necrosis factor-a (TNF-a) mō ngā rā e 2 kia whiwhi ai i ngā DC me te whakaaturanga nui o te CD11c me te CD83, he kaha ake te kaha ki te whakatairanga i te tipu o ngā pūtau CD4+T allogeneic me ngā pūtau CD8+T. He maha ngā polysaccharides mai i ngā pūtake taiao he tino pai te mahi ārai mate, pērā i ngā polysaccharides mai i ngā harore shiitake, ngā harore whatiwhati, ngā harore Yunzhi, me ngā Poria cocos, kua whakamahia i roto i ngā mahi haumanu. Ka taea e rātou te whakapai ake i te mahi ārai mate o te tinana, te whakarei ake i te ārai mate, me te mahi hei rongoā āwhina mō te maimoatanga ārai-pukupuku. Heoi, he iti noa ngā pūrongo rangahau mō te PFC hei kaiwhakatikatika ārai mate tangata. Nō reira, ka whakahaerehia e tēnei tuhinga he rangahau tuatahi mō te mahi me ngā tikanga e pā ana ki te PFC ki te whakatairanga i te pakeketanga o ngā moDC, kia taea ai te aromatawai i te kaha o te PFC hei kaiwhakatikatika ārai mate taiao.

Nā te iti rawa o te ōwehenga o ngā DC i roto i ngā kiko tangata me te nui o te tiakitanga momo i waenga i ngā DC kiore me ngā DC tangata, hei whakaoti rapanga rangahau i puta mai i te iti o te whakaputanga DC, kua rangahaua ngā tauira whakapūtanga in vitro o ngā DC i ahu mai i ngā pūtau mononuclear toto tangata, ka taea te whiwhi DC me te pai o te ārai mate i roto i te wā poto. Nō reira, i whakamahia e tēnei rangahau te tikanga tuku iho o te whakapūtanga i ngā DC tangata i roto i te vitro: te whakatipu tahi i te rhGM CSF me te rhIL-4 i roto i te vitro, te whakarerekē i te reo i ia rā, me te whiwhi DC kāore anō kia pakari i te rā 5; I te rā 6, i tāpirihia ngā rahinga ōrite o te PBS, PFC, me te LPS i runga i te whakarōpūtanga, ā, i whakapūtangahia mō te 24 hāora hei tikanga whakatipu mō te whakapūtanga i ngā DC i ahu mai i ngā pūtau mononuclear toto tangata.

Ko ngā polysaccharides i ahu mai i ngā hua taiao he iti te paitini me te iti o te utu hei whakaoho i te mate. Whai muri i ngā whakamātautau tuatahi, i kitea e tā mātou rōpū rangahau he nui te whakarei ake a te PFC i te tohu pakeke CD83 i runga i te mata o ngā pūtau DC i ahu mai i te toto tangata i roto i te vitro. I whakaatuhia e ngā hua o te flow cytometry ko te wawaotanga PFC i te kukū o te 10 μ g/mL mō ngā haora 24 i hua ake ai te whakaaturanga tihi o te tohu pakeke CD83 i runga i te mata o ngā DC, e tohu ana kua uru ngā DC ki te āhua pakeke. Nō reira, i whakatauhia e tā mātou rōpū rangahau te mahere whakaoho me te wawaotanga in vitro. Ko te CD83 he tohu koiora pakeke nui i runga i te mata o ngā DC, ko te CD86 he ngota whakaoho nui i runga i te mata o ngā DC, e mahi ana hei tohu tuarua mō te whakahohe i ngā pūtau T. Ko te whakanui ake i te whakaaturanga o ngā tohu koiora e rua, ko te CD83 me te CD86, e tohu ana ka whakatairanga te PFC i te pakeketanga o ngā DC i ahu mai i te toto tangata, e kī ana ka taea e te PFC te whakanui ake i te taumata huna o ngā cytokines i runga i te mata o ngā DC. Nō reira, i arotakehia e tēnei rangahau ngā taumata o ngā cytokine IL-6, TNF-a, me te IL-10 i tukuna e ngā DC mā te whakamahi i te ELISA. He hononga tata te IL-10 ki te manawanui o te pūnaha ārai mate o ngā DC, ā, ko ngā DC he manawanui o te pūnaha ārai mate e whakamahia whānuitia ana i roto i te maimoatanga pukupuku, e whakarato ana i ngā whakaaro rongoā pea mō te manawanui o te pūnaha ārai mate i roto i te whakawhitinga okana; He mahi nui tā te whānau 1L-6 i roto i te ārai mate ā-roto me te urutau, te hematopoiesis, me ngā pānga ārai-mumura; He rangahau e tohu ana ka whai wāhi tahi a IL-6 me TGF β ki te wehewehenga o ngā pūtau Th17; Ina whakaekea te tinana e te huaketo, ka mahi te TNF-a i hangaia e ngā DC hei whakautu ki te whakahohenga huaketo hei take pakeketanga autocrine hei whakatairanga i te pakeketanga o te DC. Mā te aukati i te TNF-a ka waiho ngā DC ki tētahi wāhanga koretake, ka aukati i a rātou ki te whakamahi katoa i tā rātou mahi whakaaturanga antigen. I whakaatuhia e ngā raraunga ELISA i roto i tēnei rangahau he nui ake te pikinga o te taumata huna o te IL-10 i roto i te rōpū PFC ki te whakataurite ki ētahi atu rōpū e rua, e tohu ana ka whakarei ake a PFC i te manawanui o te pūnaha ārai mate o ngā DC; Ko te pikinga ake o ngā taumata huna o te IL-6 me te TNF-a e kī ana tera pea ka whai pānga te PFC ki te whakarei ake i te DC hei whakatairanga i te rerekētanga o ngā pūtau-T.