Polysaccharide inobva kuChlorella (PFC), sepolysaccharide yechisikigo, yakakwezva kutariswa kukuru kubva kunyanzvi mumakore achangopfuura nekuda kwekubatsira kwayo kwechepfu shoma, migumisiro yakaderera, uye mhedzisiro ye-broad-spectrum. Mabasa ayo mukuderedza mafuta eropa, kurwisa bundu, kurwisa kuzvimba, kurwisa Parkinson's, kurwisa kukwegura, nezvimwewo zvakambosimbiswa kare mukuyedza kwe in vitro uye in vivo. Zvisinei, kuchine mukaha mukutsvaga kwePFC semugadziri wekudzivirira muviri wemunhu.

Masero eDendritic (DCs) ndiwo masero ane simba kwazvo anogadzira antigen mumuviri wemunhu. Huwandu hwemaDC mumuviri wemunhu hudiki kwazvo, uye modhi ye cytokine inoshandiswa mu vitro, inonzi human peripheral blood mononuclear cell-derived DCs (moDCs), ndiyo inowanzoshandiswa. Modhi yeDC inokonzerwa ne in vitro yakatanga kutaurwa muna 1992, inova ndiyo nzira yetsika ye DCs. Kazhinji, inoda kurimwa kwemazuva 6-7. Masero emapfupa embeva anogona kurimwa ne granulocyte macrophage colony-stimulating factor (GM-CSF) uye interleukin (IL) -4 kuti awane maDCs asina kukura (PBS group). MaCytokines anowedzerwa semastimuli akakura uye anorimwa kwemazuva 1-2 kuti awane maDCs akakura. Imwe ongororo yakataura kuti masero eCD14+ evanhu akacheneswa akarimwa neinterferon – β (IFN – β) kana IL-4 kwemazuva mashanu, uye akazorimwa ne tumor necrosis factor-a (TNF-a) kwemazuva maviri kuti awane maDC ane CD11c neCD83 yakawanda, ayo ane simba rakasimba rekusimudzira kuwanda kwemasero eCD4+T neCD8+T. Mapolysaccharides akawanda anobva kuzvisikwa ane basa rakanaka rekudzivirira muviri, senge mapolysaccharides anobva kuhowa dzeshiitake, howa dzesplit gill, howa dzeYunzhi, nePoria cocos, dzakashandiswa mukurapa. Dzinogona kuvandudza mashandiro emuviri, kuwedzera dziviriro yemuviri, uye kushanda semishonga inobatsira pakurapa bundu. Zvisinei, kune mishumo mishoma yekutsvagisa pamusoro pePFC semugadziri wedziviriro yemuviri. Saka, chinyorwa chino chinoita tsvakiridzo yekutanga pamusoro pebasa uye nzira dzakabatana dzePFC mukusimudzira kukura kwemoDCs, kuitira kuongorora kugona kwePFC semugadziri wedziviriro yemuviri wepanyama.

Nekuda kwehuwandu hushoma hwemaDC munyama dzevanhu uye kuchengetedzwa kwakawanda pakati pemaDC embeva nemaDC evanhu, kuitira kugadzirisa matambudziko ekutsvagisa anokonzerwa nekugadzirwa kwakaderera kweDC, mamodheru eDC anobva muropa revanhu akadzidzwa mu vitro, ayo anogona kuwana maDC ane simba rekudzivirira muviri munguva pfupi. Nokudaro, chidzidzo ichi chakashandisa nzira yechinyakare yekukurudzira maDC evanhu mu vitro: co culturing rhGM CSF ne rhIL-4 mu vitro, kuchinja nzira yacho zuva rega rega, uye kuwana maDC asina kukura pazuva rechishanu; Pazuva rechitanhatu, huwandu hwakaenzana hwePBS, PFC, neLPS hwakawedzerwa zvichienderana nekuunganidzwa uye kurimwa kwemaawa makumi maviri nemana senzira yekusimudzira kukurudzira maDC anobva muropa revanhu.

MaPolysaccharides anobva muzvigadzirwa zvechisikigo ane mabhenefiti ekuva nehupfu hushoma uye mutengo wakaderera se immunostimulants. Mushure mekuyedza kwekutanga, boka redu rekutsvagisa rakaona kuti PFC inowedzera zvakanyanya chiratidzo cheCD83 chakakura pamusoro pemasero eDC anowanikwa muropa revanhu anokonzerwa nemasero emunonuclear anokonzerwa ne in vitro. Mhedzisiro yeFlow cytometry yakaratidza kuti kupindira kwePFC pakuwanda kwe10 μ g/mL kwemaawa makumi maviri nemana kwakaguma nekuratidzwa kwepamusoro kwechiratidzo cheCD83 chakakura pamusoro peDCs, zvichiratidza kuti maDCs akapinda mumamiriro ekukura. Nokudaro, boka redu rekutsvagisa rakaona hurongwa hwe induction uye intervention induction. CD83 i biomarker yakakosha pamusoro peDCs, nepo CD86 inoshanda semorekuru yakakosha yekukurudzira pamusoro peDCs, ichishanda sechiratidzo chechipiri chekushanda kwemasero eT. Kuwedzerwa kwekutaura kwema biomarkers maviri CD83 neCD86 kunoratidza kuti PFC inokurudzira kukura kwemasero emunonuclear anowanikwa muropa revanhu, zvichiratidza kuti PFC inogona kuwedzera panguva imwe chete huwandu hwema cytokines pamusoro peDCs. Saka, chidzidzo ichi chakaongorora huwandu hwema cytokines IL-6, TNF-a, uye IL-10 anoburitswa neDCs vachishandisa ELISA. IL-10 ine hukama hwakasimba nekudzivirira muviri kweDCs, uye maDCs ane immune tolerance anowanzo shandiswa mukurapa bundu, zvichipa mazano ekurapa ekudzivirira muviri mukuisirwa nhengo; Mhuri ye1L-6 inoita basa rakakosha mukudzivirira muviri, hematopoiesis, uye anti-inflammatory effects; Pane zvidzidzo zvinoratidza kuti IL-6 neTGF β zvinobatana mukusiyanisa masero eTh17; Kana muviri wapinda nehutachiona, TNF-a inogadzirwa neDCs mukupindura kuvhiyiwa inoshanda se autocrine maturation factor kukurudzira DC maturation. Kuvhara TNF-a kuchaisa maDCs padanho rekusakura, zvichivadzivirira kuti vasashandise zvizere basa ravo rekuratidzira antigen. Data reELISA muchidzidzo ichi rakaratidza kuti huwandu hwekubuda kweIL-10 muboka rePFC hwakawedzera zvakanyanya zvichienzaniswa nemamwe mapoka maviri, zvichiratidza kuti PFC inowedzera immune tolerance yeDCs; Kuwedzera kwehuwandu hwekuburitswa kweIL-6 neTNF-a kunoratidza kuti PFC inogona kuva nemigumisiro yekuwedzera DC kukurudzira kusiyana kwemasero eT.