Polysaccharide los ntawm Chlorella (PFC), ua ib qho polysaccharide ntuj, tau nyiam ntau lub siab los ntawm cov kws tshawb fawb nyob rau xyoo tas los no vim nws qhov zoo ntawm kev tsis muaj tshuaj lom, kev phiv tsis tshua muaj, thiab cov teebmeem dav dav. Nws cov haujlwm hauv kev txo cov roj ntshav, tiv thaiv qog, tiv thaiv kev o, tiv thaiv Parkinson's, tiv thaiv kev laus, thiab lwm yam tau raug lees paub ua ntej hauv vitro thiab hauv vivo kev sim. Txawm li cas los xij, tseem muaj qhov sib txawv hauv kev tshawb fawb ntawm PFC ua tus neeg tiv thaiv kab mob.

Cov hlwb Dendritic (DCs) yog cov hlwb tshwj xeeb uas muaj zog tshaj plaws hauv tib neeg lub cev. Tus naj npawb ntawm cov DCs hauv tib neeg lub cev yog me heev, thiab tus qauv cytokine mediated hauv vitro induction, uas yog tib neeg peripheral ntshav mononuclear cell-derived DCs (moDCs), feem ntau siv. Tus qauv in vitro induced DC tau tshaj tawm thawj zaug hauv xyoo 1992, uas yog lub kaw lus kab lis kev cai rau DCs. Feem ntau, nws yuav tsum tau cog rau 6-7 hnub. Cov hlwb pob txha nas tuaj yeem cog nrog granulocyte macrophage colony-stimulating factor (GM-CSF) thiab interleukin (IL) -4 kom tau txais cov DCs tsis paub tab (PBS pawg). Cytokines raug ntxiv ua cov tshuaj stimuli laus thiab cog rau 1-2 hnub kom tau txais cov DCs laus. Lwm txoj kev tshawb fawb tau tshaj tawm tias cov hlwb CD14+ uas tau ntxuav lawm tau cog nrog interferon – β (IFN – β) lossis IL-4 rau 5 hnub, thiab tom qab ntawd cog nrog tumor necrosis factor-a (TNF-a) rau 2 hnub kom tau DCs nrog kev qhia siab ntawm CD11c thiab CD83, uas muaj peev xwm txhawb kev loj hlob ntawm cov hlwb CD4+T allogeneic thiab CD8+T. Ntau cov polysaccharides los ntawm cov chaw ntuj muaj cov haujlwm immunomodulatory zoo heev, xws li polysaccharides los ntawm cov nceb shiitake, cov nceb split gill, cov nceb Yunzhi, thiab Poria cocos, uas tau siv rau hauv kev kho mob. Lawv tuaj yeem txhim kho lub cev tiv thaiv kab mob, txhim kho kev tiv thaiv kab mob, thiab ua haujlwm ua cov kev kho mob adjuvant rau kev kho mob qog. Txawm li cas los xij, muaj ob peb daim ntawv tshawb fawb txog PFC ua tus neeg tiv thaiv kab mob. Yog li ntawd, tsab xov xwm no ua qhov kev tshawb fawb ua ntej txog lub luag haujlwm thiab cov txheej txheem cuam tshuam ntawm PFC hauv kev txhawb nqa kev loj hlob ntawm moDCs, txhawm rau ntsuas qhov muaj peev xwm ntawm PFC ua tus neeg tiv thaiv kab mob ntuj.

Vim yog qhov sib piv tsawg heev ntawm DCs hauv tib neeg cov nqaij thiab kev txuag ntau hom ntawm nas DCs thiab tib neeg DCs, txhawm rau daws qhov teeb meem kev tshawb fawb los ntawm kev tsim DC tsawg, cov qauv induction hauv vitro ntawm DCs uas tau los ntawm tib neeg cov ntshav peripheral mononuclear hlwb tau raug kawm, uas tuaj yeem tau txais DCs nrog kev tiv thaiv zoo hauv lub sijhawm luv luv. Yog li ntawd, txoj kev tshawb fawb no siv txoj kev ib txwm ntawm kev inducing tib neeg DCs hauv vitro: co culturing rhGM CSF thiab rhIL-4 hauv vitro, hloov cov khoom nruab nrab txhua lwm hnub, thiab tau txais cov DCs tsis paub tab rau hnub 5; Nyob rau hnub 6, sib npaug ntawm PBS, PFC, thiab LPS tau ntxiv raws li kev sib sau ua ke thiab cog qoob loo rau 24 teev ua cov txheej txheem kab lis kev cai rau inducing DCs uas tau los ntawm tib neeg cov ntshav peripheral mononuclear hlwb.

Cov polysaccharides uas tau los ntawm cov khoom ntuj muaj qhov zoo ntawm kev tsis muaj tshuaj lom thiab tus nqi qis ua cov tshuaj immunostimulants. Tom qab kev sim ua ntej, peb pawg tshawb fawb pom tias PFC ua rau cov cim CD83 loj hlob zoo dua ntawm qhov chaw ntawm cov ntshav peripheral mononuclear cell-derived DC cells uas tau tshwm sim hauv vitro. Cov txiaj ntsig ntawm Flow cytometry qhia tau hais tias PFC kev cuam tshuam ntawm qhov concentration ntawm 10 μ g / mL rau 24 teev ua rau muaj qhov siab tshaj plaws ntawm cov cim CD83 loj hlob ntawm qhov chaw ntawm DCs, qhia tias DCs nkag mus rau hauv lub xeev loj hlob. Yog li ntawd, peb pawg tshawb fawb tau txiav txim siab qhov kev npaj induction thiab kev cuam tshuam hauv vitro. CD83 yog ib qho tseem ceeb biomarker loj hlob ntawm qhov chaw ntawm DCs, thaum CD86 ua haujlwm ua ib qho tseem ceeb co stimulatory molecule ntawm qhov chaw ntawm DCs, ua lub cim thib ob rau kev ua kom T cell ua haujlwm. Kev qhia ntxiv ntawm ob lub biomarkers CD83 thiab CD86 qhia tau hais tias PFC txhawb kev loj hlob ntawm cov ntshav peripheral mononuclear cell-derived DCs, qhia tias PFC tuaj yeem ua rau cov cytokines tso tawm ntawm qhov chaw ntawm DCs tib lub sijhawm. Yog li ntawd, txoj kev tshawb fawb no tau soj ntsuam cov theem ntawm cytokines IL-6, TNF-a, thiab IL-10 uas DCs tso tawm siv ELISA. IL-10 muaj feem cuam tshuam nrog kev tiv thaiv kab mob ntawm DCs, thiab DCs uas muaj kev tiv thaiv kab mob feem ntau siv rau hauv kev kho mob qog, muab cov tswv yim kho mob rau kev tiv thaiv kab mob hauv kev hloov pauv lub cev; Tsev neeg 1L-6 ua lub luag haujlwm tseem ceeb hauv kev tiv thaiv kab mob thiab kev hloov pauv, hematopoiesis, thiab cov teebmeem tiv thaiv kev o; Muaj cov kev tshawb fawb qhia tias IL-6 thiab TGF β koom ua ke hauv kev sib txawv ntawm Th17 hlwb; Thaum lub cev raug tus kab mob nkag mus, TNF-a tsim los ntawm DCs los teb rau kev ua haujlwm ntawm tus kab mob ua haujlwm ua tus yam ntxwv autocrine maturation los txhawb DC maturation. Kev thaiv TNF-a yuav muab DCs tso rau hauv theem tsis paub tab, tiv thaiv lawv los ntawm kev siv lawv cov haujlwm nthuav qhia antigen tag nrho. Cov ntaub ntawv ELISA hauv txoj kev tshawb fawb no qhia tau hais tias qib secretion ntawm IL-10 hauv pawg PFC tau nce ntxiv ntau piv rau lwm ob pawg, qhia tias PFC txhim kho kev tiv thaiv kab mob ntawm DCs; Qhov kev tso tawm ntawm IL-6 thiab TNF-a ntau ntxiv qhia tias PFC yuav muaj qhov cuam tshuam ntawm kev txhim kho DC los txhawb kev sib txawv ntawm T cell.