Polysaccharide yochokera ku Chlorella (PFC), monga polysaccharide yachilengedwe, yakopa chidwi chachikulu kuchokera kwa akatswiri m'zaka zaposachedwa chifukwa cha ubwino wake wa poizoni wochepa, zotsatira zake zochepa, komanso zotsatira zake zazikulu. Ntchito zake pochepetsa mafuta m'magazi, zotsutsana ndi chotupa, zotsutsana ndi kutupa, zotsutsana ndi Parkinson's, zotsutsana ndi ukalamba, ndi zina zotero zatsimikiziridwa kale mu kafukufuku wa in vitro ndi in vivo. Komabe, pakadalibe kusiyana mu kafukufuku wa PFC ngati modulator ya chitetezo chamthupi cha munthu.

Maselo a dendritic (DCs) ndi maselo amphamvu kwambiri omwe amapereka ma antigen m'thupi la munthu. Chiwerengero cha ma DC m'thupi la munthu ndi chochepa kwambiri, ndipo chitsanzo cha cytokine chomwe chimayendetsedwa ndi in vitro, chomwe ndi ma DC (moDCs) omwe amapezeka m'magazi a anthu, chimagwiritsidwa ntchito kwambiri. Chitsanzo cha DC chomwe chimagwiritsidwa ntchito mu in vitro chinanenedwa koyamba mu 1992, chomwe ndi njira yachikhalidwe yophunzitsira ma DC. Nthawi zambiri, chimafunika kulimidwa kwa masiku 6-7. Maselo a mbewa amatha kulimidwa ndi granulocyte macrophage colony-stimulating factor (GM-CSF) ndi interleukin (IL) -4 kuti apeze ma DC osakhwima (gulu la PBS). Ma Cytokines amawonjezedwa ngati zinthu zokhwima ndipo amalimidwa kwa masiku 1-2 kuti apeze ma DC okhwima. Kafukufuku wina adanenanso kuti maselo a CD14+a anthu oyera adalimidwa ndi interferon - β (IFN - β) kapena IL-4 kwa masiku 5, kenako adalimidwa ndi tumor necrosis factor-a (TNF-a) kwa masiku awiri kuti apeze ma DC okhala ndi kuchuluka kwa CD11c ndi CD83, omwe ali ndi mphamvu yayikulu yolimbikitsa kuchuluka kwa maselo a CD4+T a allogeneic ndi maselo a CD8+T. Ma polysaccharide ambiri ochokera kuzinthu zachilengedwe ali ndi mphamvu yabwino kwambiri yowongolera chitetezo chamthupi, monga ma polysaccharide ochokera ku bowa wa shiitake, bowa wa split gill, bowa wa Yunzhi, ndi Poria cocos, omwe agwiritsidwa ntchito kuchipatala. Angathe kupititsa patsogolo ntchito ya chitetezo chamthupi, kulimbitsa chitetezo chamthupi, komanso kukhala ngati mankhwala othandizira pochiza chotupa. Komabe, pali malipoti ochepa ofufuza pa PFC ngati modulator ya chitetezo chamthupi cha anthu. Chifukwa chake, nkhaniyi ikuchita kafukufuku woyambirira pa ntchito ndi njira zina zokhudzana ndi PFC pakulimbikitsa kukula kwa ma moDC, kuti tiwone momwe PFC ingathandizire ngati modulator yachilengedwe ya chitetezo chamthupi.

Chifukwa cha kuchuluka kochepa kwambiri kwa ma DC m'thupi la munthu komanso kusungidwa kwakukulu kwa mitundu pakati pa ma DC a mbewa ndi ma DC a anthu, kuti athetse mavuto ofufuza omwe amabwera chifukwa cha kupanga kochepa kwa ma DC, mitundu yoyambira ya ma DC ochokera ku maselo a mononuclear am'magazi a anthu yaphunziridwa, yomwe ingapeze ma DC okhala ndi chitetezo chamthupi chabwino pakapita nthawi yochepa. Chifukwa chake, kafukufukuyu adagwiritsa ntchito njira yachikhalidwe yolimbikitsira ma DC a anthu mu vitro: kuphatikiza rhGM CSF ndi rhIL-4 mu vitro, kusintha njira tsiku lililonse, ndikupeza ma DC osakhwima pa tsiku la 5; Pa tsiku la 6, kuchuluka kofanana kwa PBS, PFC, ndi LPS kunawonjezedwa malinga ndi magulu ndi kulimidwa kwa maola 24 ngati njira yolimbikitsira kulimbikitsira ma DC ochokera ku maselo a mononuclear am'magazi a anthu.

Ma polysaccharide ochokera ku zinthu zachilengedwe ali ndi ubwino wa poizoni wotsika komanso mtengo wotsika ngati ma immunostimulants. Pambuyo pa kuyesa koyambirira, gulu lathu lofufuza linapeza kuti PFC imakulitsa kwambiri chizindikiro chokhwima cha CD83 pamwamba pa maselo a DC omwe amachokera ku magazi a anthu omwe amachokera ku maselo a DC omwe amachokera ku maselo a m'magazi. Zotsatira za flow cytometry zinawonetsa kuti kulowererapo kwa PFC pa kuchuluka kwa 10 μ g/mL kwa maola 24 kunapangitsa kuti chizindikiro chokhwima cha CD83 chiwoneke pamwamba pa DCs, zomwe zikusonyeza kuti ma DCs adalowa mu mkhalidwe wokhwima. Chifukwa chake, gulu lathu lofufuza linapeza dongosolo loyambitsa ndi kulowererapo mu vitro. CD83 ndi biomarker yofunika kwambiri pamwamba pa DCs, pomwe CD86 imagwira ntchito ngati molekyulu yofunika kwambiri yolimbikitsa pamwamba pa DCs, ikugwira ntchito ngati chizindikiro chachiwiri choyambitsa maselo a T. Kuwonjezeka kwa ma biomarker awiri a CD83 ndi CD86 kukuwonetsa kuti PFC imalimbikitsa kukhwima kwa ma DCs omwe amachokera ku maselo a m'magazi a anthu, zomwe zikusonyeza kuti PFC ikhoza kuwonjezera nthawi imodzi kuchuluka kwa ma cytokines pamwamba pa DCs. Chifukwa chake, kafukufukuyu adawunika kuchuluka kwa ma cytokines IL-6, TNF-a, ndi IL-10 omwe amatulutsidwa ndi ma DC pogwiritsa ntchito ELISA. IL-10 imagwirizana kwambiri ndi kulekerera kwa chitetezo cha mthupi kwa ma DC, ndipo ma DC omwe ali ndi kulekerera kwa chitetezo cha mthupi amagwiritsidwa ntchito kwambiri pochiza chotupa, kupereka malingaliro ochiritsira omwe angathe kukhala olekerera chitetezo cha mthupi pakuika ziwalo; Banja la 1L-6 limagwira ntchito yofunika kwambiri pa chitetezo chamthupi chobadwa nacho komanso chosinthika, hematopoiesis, ndi zotsatira zotsutsana ndi kutupa; Pali maphunziro omwe akusonyeza kuti IL-6 ndi TGF β zimagwirizana pakusiyanitsa maselo a Th17; Thupi likalowa ndi kachilombo, TNF-a yopangidwa ndi ma DC poyankha kuyambitsa kachilombo imagwira ntchito ngati chinthu chokhwima cha autocrine kuti ilimbikitse kukula kwa DC. Kuletsa TNF-a kudzaika ma DC mu gawo losakhwima, kuwaletsa kugwiritsa ntchito mokwanira ntchito yawo yowonetsera antigen. Deta ya ELISA mu kafukufukuyu idawonetsa kuti kuchuluka kwa IL-10 m'gulu la PFC kudakwera kwambiri poyerekeza ndi magulu ena awiri, zomwe zikusonyeza kuti PFC imakulitsa kulekerera kwa chitetezo chamthupi kwa ma DC; Kuwonjezeka kwa kuchuluka kwa IL-6 ndi TNF-a kukuwonetsa kuti PFC ikhoza kukhala ndi mphamvu yowonjezera DC kuti ipititse patsogolo kusiyana kwa maselo a T.