Polysaccharide sitere na Chlorella (PFC), dịka polysaccharide eke, adọtala ọtụtụ ndị ọkà mmụta n'afọ ndị na-adịbeghị anya n'ihi uru ya dị na obere nsí, obere mmetụta ọjọọ, na mmetụta sara mbara. Ọrụ ya na ibelata lipids ọbara, mgbochi etuto ahụ, mgbochi mbufụt, mgbochi Parkinson, mgbochi ịka nká, wdg. A ka kwadoro nnwale in vitro na in vivo. Agbanyeghị, enwere oghere na nyocha na PFC dị ka ihe na-eme ka ahụ mmadụ ghara ịlụso ọrịa ọgụ.

Mkpụrụ ndụ Dendritic (DCs) bụ mkpụrụ ndụ kachasị ike nke na-enye antigen n'ahụ mmadụ. Ọnụọgụ DC dị n'ahụ mmadụ dị obere nke ukwuu, a na-ejikarịkwa ụdị cytokine nke na-eme ka mmadụ nwee ike itinye n'ime ahụ, ya bụ, DCs sitere na mkpụrụ ndụ mononuclear ọbara (moDCs). A kọrọ na mbụ ụdị DC nke in vitro kpaliri na 1992, nke bụ usoro omenala ọdịnala maka DCs. N'ozuzu, ọ chọrọ ịkụ ihe ruo ụbọchị 6-7. Enwere ike ịkụ mkpụrụ ndụ ọkpụkpụ òké na granulocyte macrophage colony-stimulating factor (GM-CSF) na interleukin (IL) -4 iji nweta DCs na-etobeghị eto (PBS group). A na-agbakwụnye Cytokines dị ka ihe mkpali tozuru okè ma na-akọ ha maka ụbọchị 1-2 iji nweta DCs tozuru okè. Nnyocha ọzọ kọrọ na e ji interferon – β (IFN – β) ma ọ bụ IL-4 kụkọta mkpụrụ ndụ CD14+ mmadụ dị ọcha ruo ụbọchị ise, wee jiri tumor necrosis factor-a (TNF-a) kụọ mkpụrụ ndụ abụọ iji nweta DCs nwere oke ngosipụta nke CD11c na CD83, nke nwere ike ịkwalite mmụba nke mkpụrụ ndụ allogeneic CD4+T na mkpụrụ ndụ CD8+T. Ọtụtụ polysaccharides sitere na isi mmalite eke nwere ọrụ immunomodulatory dị mma, dị ka polysaccharides sitere na ero shiitake, ero split gill, ero Yunzhi, na Poria cocos, nke etinyere na omume ahụike. Ha nwere ike imeziwanye ọrụ ahụ ji alụso ọrịa ọgụ nke ọma, melite mgbochi ọrịa, ma jee ozi dị ka ọgwụgwọ adjuvant maka ọgwụgwọ mgbochi ọrịa. Agbanyeghị, enwere akụkọ nyocha ole na ole na PFC dị ka ihe na-akwalite mgbochi ọrịa mmadụ. Ya mere, isiokwu a na-eme nnyocha mbụ na ọrụ na usoro ndị metụtara ya nke PFC na-akwalite ntozu oke nke moDCs, iji nyochaa ike nke PFC dị ka ihe na-akwalite mgbochi ọrịa.

N'ihi oke oke nke DCs n'ime anụ ahụ mmadụ na oke nchekwa dị n'etiti oke DCs na DCs mmadụ, iji dozie nsogbu nyocha nke obere mmepụta DC kpatara, amụọla ụdị induction in vitro nke DCs sitere na mkpụrụ ndụ mononuclear ọbara mmadụ, nke nwere ike inweta DCs nwere ezigbo immunogenicity n'ime obere oge. Ya mere, ọmụmụ ihe a jiri usoro ọdịnala nke ịkpalite DCs mmadụ na vitro: ịkpalite rhGM CSF na rhIL-4 na vitro, ịgbanwe usoro kwa ụbọchị abụọ, na inweta DCs na-etobeghị eto n'ụbọchị nke ise; N'ụbọchị nke isii, etinyere nha nhata nke PBS, PFC, na LPS dịka nhazi ma zụlite ya ruo awa 24 dị ka usoro omenala maka ịkpalite DCs sitere na mkpụrụ ndụ mononuclear ọbara mmadụ.

Polysaccharides sitere na ngwaahịa eke nwere uru nke obere nsí na ọnụ ala dị ka ihe na-akpali immunostimulants. Mgbe nnwale mbụ gasịrị, otu nnyocha anyị chọpụtara na PFC na-eme ka akara CD83 tozuru okè dịkwuo elu n'elu mkpụrụ ndụ DC sitere na mkpụrụ ndụ mononuclear nke mmadụ kpatara na in vitro. Nsonaazụ cytometry flow gosiri na ntinye aka PFC na mkpokọta nke 10 μ g/mL ruo awa 24 butere ngosipụta kachasị elu nke akara CD83 tozuru okè n'elu DCs, na-egosi na DCs banyere n'ọnọdụ tozuru okè. Ya mere, otu nyocha anyị kpebiri atụmatụ ntinye na ntinye aka na in vitro. CD83 bụ ihe ngosi biomarker dị mkpa n'elu DCs, ebe CD86 na-eje ozi dị ka molekul co-stimulatory dị mkpa n'elu DCs, na-arụ ọrụ dị ka ihe mgbaàmà nke abụọ maka ịgbalite mkpụrụ ndụ T. Ngosipụta ka mma nke ihe ngosi biomarker abụọ CD83 na CD86 na-egosi na PFC na-akwalite ntozu nke DCs sitere na mkpụrụ ndụ mononuclear ọbara mmadụ, na-atụ aro na PFC nwere ike ịbawanye ọkwa secretion nke cytokines n'elu DCs n'otu oge. Ya mere, ọmụmụ ihe a tụlere ọkwa nke cytokines IL-6, TNF-a, na IL-10 nke DCs na-ewepụta site na iji ELISA. IL-10 nwere njikọ chiri anya na nnabata ahụ ike nke DCs, a na-ejikwa DCs nwere ndidi ahụ ike na ọgwụgwọ etuto ahụ, na-enye echiche ọgwụgwọ nwere ike maka nnabata ahụ ike na ntụgharị ahụ; Ezinụlọ 1L-6 na-arụ ọrụ dị mkpa na mgbochi ahụ ike nke ebumpụta ụwa na nke na-agbanwe agbanwe, hematopoiesis, na mmetụta mgbochi mkpali; E nwere ọmụmụ ihe na-egosi na IL-6 na TGF β na-esonye na nhazi nke mkpụrụ ndụ Th17; Mgbe nje virus wakporo ahụ, TNF-a nke DCs mepụtara na nzaghachi maka mmegharị ahụ nje na-arụ ọrụ dị ka ihe na-eme ka autocrine tolite iji kwalite ntozu oke DC. Igbochi TNF-a ga-etinye DCs n'ọkwa na-etobeghị eto, na-egbochi ha ịrụ ọrụ ngosipụta antigen ha zuru oke. Data ELISA dị na ọmụmụ ihe a gosiri na ọkwa nzuzo nke IL-10 na otu PFC mụbara nke ukwuu ma e jiri ya tụnyere otu abụọ ndị ọzọ, na-egosi na PFC na-eme ka nnabata ahụ ike nke DCs dịkwuo mma; Ọkwa IL-6 na TNF-a na-abawanye nke na-egosi na PFC nwere ike inwe mmetụta nke ime ka DC dịkwuo mma iji kwalite ọdịiche mkpụrụ ndụ T.