Polysaccharide daga Chlorella (PFC), a matsayin polysaccharide na halitta, ya jawo hankalin masana sosai a cikin 'yan shekarun nan saboda fa'idodinsa na ƙarancin guba, ƙarancin illa, da kuma tasirin da ke da faɗi. Ayyukansa wajen rage kitse a cikin jini, hana ƙari, hana kumburi, hana Parkinson's, hana tsufa, da sauransu an riga an tabbatar da su a gwaje-gwajen in vitro da in vivo. Duk da haka, har yanzu akwai gibi a cikin binciken da aka yi kan PFC a matsayin mai daidaita garkuwar jiki na ɗan adam.

Kwayoyin Dendritic (DCs) su ne ƙwayoyin halitta mafi ƙarfi waɗanda ke gabatar da antigen a jikin ɗan adam. Adadin DCs a jikin ɗan adam ƙanƙanta ne ƙwarai, kuma ana amfani da samfurin cytokine mai shiga tsakani a cikin vitro, wato DCs na ƙwayoyin mononuclear na jini na ɗan adam (moDCs), wanda aka samo daga ƙwayoyin halitta na ɗan adam (moDCs). An fara bayar da rahoton samfurin DC da aka haifar a cikin vitro a cikin 1992, wanda shine tsarin al'ada na gargajiya ga DCs. Gabaɗaya, yana buƙatar noma na tsawon kwanaki 6-7. Ana iya haɓaka ƙwayoyin ƙashi na beraye da granulocyte macrophage colony-stimulating factor (GM-CSF) da interleukin (IL) -4 don samun DCs marasa girma (ƙungiyar PBS). Ana ƙara Cytokines a matsayin abubuwan ƙarfafawa na manya kuma ana haɓaka su na tsawon kwanaki 1-2 don samun DCs masu girma. Wani bincike ya ruwaito cewa an yi wa ƙwayoyin CD14+ na ɗan adam da aka tsarkake da interferon – β (IFN – β) ko IL-4 na tsawon kwanaki 5, sannan a yi musu da tumor necrosis factor-a (TNF-a) na tsawon kwanaki 2 don samun DCs masu yawan CD11c da CD83, waɗanda ke da ƙarfin ƙarfafa yaduwar ƙwayoyin CD4+T allogeneic da ƙwayoyin CD8+T. Polysaccharides da yawa daga tushen halitta suna da kyakkyawan aikin immunomodulatory, kamar polysaccharides daga namomin kaza shiitake, namomin kaza split gill, namomin kaza Yunzhi, da Poria cocos, waɗanda aka yi amfani da su a aikin asibiti. Suna iya inganta aikin garkuwar jiki yadda ya kamata, haɓaka rigakafi, da kuma zama magunguna masu taimako don maganin ciwon daji. Duk da haka, akwai rahotannin bincike kaɗan game da PFC a matsayin mai daidaita garkuwar jiki na ɗan adam. Saboda haka, wannan labarin yana gudanar da bincike na farko kan rawar da PFC ke takawa wajen haɓaka balaga na moDCs, don kimanta yuwuwar PFC a matsayin mai daidaita garkuwar jiki na halitta.

Saboda ƙarancin adadin DCs a cikin kyallen ɗan adam da kuma yawan kiyayewa tsakanin nau'ikan halittu tsakanin beraye DCs da beraye DCs, domin magance matsalolin bincike da ƙarancin samar da DC ke haifarwa, an yi nazarin samfuran induction na DCs da aka samo daga ƙwayoyin mononuclear na jini na ɗan adam, waɗanda za su iya samun DCs masu ingantaccen rigakafi cikin ɗan gajeren lokaci. Saboda haka, wannan binciken ya yi amfani da hanyar gargajiya ta haifar da DCs na ɗan adam a cikin vitro: haɓaka rhGM CSF da rhIL-4 a cikin vitro, canza yanayin kowace rana, da kuma samun DCs marasa girma a rana ta 5; A rana ta 6, an ƙara adadin PBS, PFC, da LPS daidai gwargwado kuma an haɓaka su na tsawon awanni 24 a matsayin tsarin al'ada don haifar da DCs da aka samo daga ƙwayoyin mononuclear na jini na ɗan adam.

Polysaccharides da aka samo daga samfuran halitta suna da fa'idodin ƙarancin guba da ƙarancin farashi a matsayin masu ƙarfafa garkuwar jiki. Bayan gwaje-gwaje na farko, ƙungiyar bincikenmu ta gano cewa PFC tana haɓaka alamar girma CD83 sosai akan saman ƙwayoyin DC da aka samo daga ƙwayoyin mononuclear na jini na ɗan adam da aka haifar a cikin vitro. Sakamakon kwararar cytometry ya nuna cewa shiga tsakani na PFC a cikin taro na 10 μg/mL na tsawon awanni 24 ya haifar da mafi girman bayyanar alamar girma CD83 akan saman DCs, yana nuna cewa DCs sun shiga yanayin girma. Saboda haka, ƙungiyar bincikenmu ta ƙayyade tsarin shigarwa da shiga tsakani na in vitro. CD83 muhimmin alamar girma ne akan saman DCs, yayin da CD86 ke aiki a matsayin muhimmin ƙwayar ƙarfafawa akan saman DCs, yana aiki a matsayin sigina na biyu don kunna ƙwayoyin T. Ingantaccen bayyanar alamun biomarkers guda biyu CD83 da CD86 yana nuna cewa PFC yana haɓaka balaga na DCs da aka samo daga ƙwayoyin mononuclear na jini na ɗan adam, yana nuna cewa PFC na iya ƙara matakin ɓoyewar cytokines a saman DCs a lokaci guda. Saboda haka, wannan binciken ya kimanta matakan cytokines IL-6, TNF-a, da IL-10 da DCs suka fitar ta amfani da ELISA. IL-10 yana da alaƙa da juriyar garkuwar jiki na DCs, kuma DCs masu juriyar garkuwar jiki ana amfani da su sosai a cikin maganin ƙari, suna ba da damar ra'ayoyin magani don jure wa garkuwar jiki a cikin dashen gabobi; Iyalin 1L-6 suna taka muhimmiyar rawa a cikin rigakafi na asali da na daidaitawa, hematopoiesis, da tasirin hana kumburi; Akwai bincike da ke nuna cewa IL-6 da TGF β suna shiga tare cikin bambance-bambancen ƙwayoyin Th17; Lokacin da kwayar cuta ta mamaye jiki, TNF-a da DCs ke samarwa a matsayin abin da ke haifar da haɓakar ƙwayar cuta don haɓaka balaga ta DC. Toshe TNF-a zai sanya DCs a cikin matakin da ba su kai ba, yana hana su yin cikakken aikin gabatar da antigen ɗinsu. Bayanan ELISA a cikin wannan binciken sun nuna cewa matakin fitar da IL-10 a cikin ƙungiyar PFC ya ƙaru sosai idan aka kwatanta da sauran ƙungiyoyi biyu, yana nuna cewa PFC yana haɓaka juriyar garkuwar jiki na DCs; Ƙara yawan fitar da IL-6 da TNF-a yana nuna cewa PFC na iya samun tasirin inganta DC don haɓaka bambance-bambancen ƙwayoyin T.