Polysaccharide e tsoang ho Chlorella (PFC), e le polysaccharide ea tlhaho, e hohetse tlhokomelo e kholo ho litsebi lilemong tsa morao tjena ka lebaka la melemo ea eona ea chefo e tlase, litla-morao tse tlase, le litlamorao tse pharaletseng. Mesebetsi ea eona ea ho fokotsa mafura maling, ho loantša hlahala, ho thibela ho ruruha, ho thibela Parkinson's, ho thibela botsofali, jj. e netefalitsoe pele litekong tsa in vitro le in vivo. Leha ho le joalo, ho ntse ho e-na le sekheo lipatlisisong tsa PFC e le modulator ea 'mele oa motho.

Lisele tsa dendritic (DCs) ke lisele tse matla ka ho fetisisa tse hlahisang antigen 'meleng oa motho. Palo ea li-DC 'meleng oa motho e nyane haholo, 'me mohlala oa induction o tsamaisoang ke cytokine in vitro, e leng li-DC tse tsoang maling a motho a ka ntle ho peripheral blood mononuclear cell-derived DCs (moDCs), o sebelisoa hangata. Mohlala oa DC o hlahisitsoeng ka in vitro o ile oa tlalehoa ka lekhetlo la pele ka 1992, e leng mokhoa oa setso oa ho lema bakeng sa li-DCs. Ka kakaretso, o hloka ho lema matsatsi a 6-7. Lisele tsa moko oa lesapo la mouse li ka lengoa ka granulocyte macrophage colony-stimulating factor (GM-CSF) le interleukin (IL) -4 ho fumana li-DCs tse sa butsoang (sehlopha sa PBS). Li-cytokine li eketsoa e le lintho tse susumetsang tse butsoitseng 'me li lengoa matsatsi a 1-2 ho fumana li-DCs tse butsoitseng. Phuputso e 'ngoe e tlalehile hore lisele tsa CD14+ tsa batho tse hloekisitsoeng li ile tsa lengoa ka interferon – β (IFN – β) kapa IL-4 matsatsi a 5, ebe li lengoa ka tumor necrosis factor-a (TNF-a) matsatsi a 2 ho fumana li-DC tse nang le tlhahiso e phahameng ea CD11c le CD83, tse nang le bokhoni bo matla ba ho khothaletsa ho ata ha lisele tsa CD4+T tsa allogeneic le lisele tsa CD8+T. Li-polysaccharide tse ngata tse tsoang mehloling ea tlhaho li na le ts'ebetso e ntle ea ho laola sesole sa 'mele, joalo ka li-polysaccharide tse tsoang ho li-mushroom tsa shiitake, li-mushroom tsa split gill, li-mushroom tsa Yunzhi le li-coco tsa Poria, tse sebelisitsoeng ts'ebetsong ea bongaka. Li ka ntlafatsa ts'ebetso ea 'mele ea ho itšireletsa mafung ka katleho, tsa ntlafatsa sesole sa 'mele,' me tsa sebetsa e le mekhoa e thusang bakeng sa kalafo ea ho thibela hlahala. Leha ho le joalo, ho na le litlaleho tse fokolang tsa lipatlisiso mabapi le PFC e le modulator ea 'mele ea ho itšireletsa mafung ea motho. Ka hona, sengoloa sena se etsa lipatlisiso tsa pele mabapi le karolo le mekhoa e amanang le PFC ho ntšetseng pele kholo ea li-moDC, e le ho lekola bokhoni ba PFC e le modulator ea tlhaho ea 'mele ea ho itšireletsa mafung.

Ka lebaka la karolo e tlase haholo ea li-DC liseleng tsa batho le paballo e phahameng ea mefuta e fapaneng lipakeng tsa li-DC tsa mouse le li-DC tsa batho, e le ho rarolla mathata a lipatlisiso a bakoang ke tlhahiso e tlase ea DC, mehlala ea induction ea li-DC tse nkiloeng liseleng tsa mononuclear tsa mali a motho e potileng e ithutiloe, e ka fumanang li-DC tse nang le immunogenicity e ntle ka nako e khuts'oane. Ka hona, phuputso ena e sebelisitse mokhoa oa setso oa ho susumetsa li-DC tsa batho ka har'a vitro: ho lema hammoho rhGM CSF le rhIL-4 ka har'a vitro, ho fetola mokhoa o mong le o mong letsatsi le leng le le leng, le ho fumana li-DC tse sa butsoang ka letsatsi la bo5; Ka letsatsi la bo6, lipalo tse lekanang tsa PBS, PFC, le LPS li ile tsa eketsoa ho latela lihlopha le ho lengoa ka lihora tse 24 e le mokhoa oa setso oa ho susumetsa li-DC tse nkiloeng liseleng tsa mononuclear tsa mali a motho a potileng.

Li-polysaccharide tse nkiloeng lihlahisoa tsa tlhaho li na le melemo ea chefo e tlase le litšenyehelo tse tlase e le li-immunostimulant. Kamora liteko tsa pele, sehlopha sa rona sa lipatlisiso se fumane hore PFC e ntlafatsa haholo letšoao le butsoitseng la CD83 holim'a lisele tsa DC tse tsoang maling a motho tse tsoang liseleng tsa DC tse hlahisitsoeng ka har'a vitro. Liphetho tsa cytometry ea phallo li bontšitse hore ho kenella ha PFC ka har'a khatello ea 10 μ g/mL ka lihora tse 24 ho hlahisitse polelo e phahameng ea letšoao le butsoitseng la CD83 holim'a DC, ho bontšang hore li-DC li kene boemong ba ho butsoitseng. Ka hona, sehlopha sa rona sa lipatlisiso se fumane moralo oa ho kenya le ho kenella ka har'a vitro. CD83 ke biomarker ea bohlokoa e butsoitseng holim'a DC, ha CD86 e sebetsa e le molek'hule ea bohlokoa e susumetsang holim'a DC, e sebetsa e le lets'oao la bobeli la ho kenya tšebetsong lisele tsa T. Pontšo e ntlafalitsoeng ea li-biomarker tse peli tsa CD83 le CD86 e bontša hore PFC e khothalletsa ho hola ha li-DC tse tsoang maling a motho tse tsoang liseleng tsa DC, e leng se bontšang hore PFC e ka eketsa boemo ba ho ntša li-cytokine holim'a DC ka nako e le 'ngoe. Ka hona, phuputso ena e hlahlobile maemo a li-cytokine IL-6, TNF-a, le IL-10 tse ntšoang ke li-DC li sebelisa ELISA. IL-10 e amana haufi-ufi le mamello ea 'mele ea li-DC, 'me li-DC tse nang le mamello ea 'mele ea li-immune li sebelisoa haholo kalafong ea hlahala, li fana ka mehopolo e ka bang teng ea phekolo ea mamello ea 'mele ea li-immune ho fetisetsoeng ha litho; Lelapa la 1L-6 le bapala karolo ea bohlokoa ho itšireletseng ha 'mele ka tlhaho le ho ikamahanya le maemo, hematopoiesis, le litlamorao tsa ho thibela ho ruruha; Ho na le lithuto tse bontšang hore IL-6 le TGF β li kopanela hammoho ho qhalaneng ha lisele tsa Th17; Ha 'mele o hlaseloa ke vaerase, TNF-a e hlahisoang ke li-DC ho arabela ts'ebetsong ea vaerase e sebetsa e le ntlha ea ho hola ha autocrine ho khothaletsa ho hola ha DC. Ho thibela TNF-a ho tla beha li-DC boemong ba ho se hole, ho li thibela ho sebelisa ka botlalo mosebetsi oa tsona oa tlhahiso ea antigen. Lintlha tsa ELISA thutong ena li bontšitse hore boemo ba ho ntša ha IL-10 sehlopheng sa PFC bo eketsehile haholo ha bo bapisoa le lihlopha tse ling tse peli, ho bontšang hore PFC e eketsa mamello ea 'mele ea li-DC; Maemo a ntseng a eketseha a tlhahiso ea IL-6 le TNF-a a bontša hore PFC e ka ba le phello ea ho ntlafatsa DC ho khothaletsa phapang ea lisele tsa T.