I-Polysaccharide evela ku-Chlorella (PFC), njenge-polysaccharide yemvelo, idonsele ukunaka okukhulu kososayensi eminyakeni yamuva ngenxa yezinzuzo zayo zobuthi obuphansi, imiphumela emibi ephansi, kanye nemiphumela ye-spectrum ebanzi. Imisebenzi yayo ekwehliseni amafutha egazini, ukulwa nomdlavuza, ukulwa nokuvuvukala, ukulwa noParkinson's, ukulwa nokuguga, njll. iye yaqinisekiswa ekuqaleni ekuhlolweni kwe-in vitro kanye ne-in vivo. Kodwa-ke, kusekhona igebe ocwaningweni lwe-PFC njenge-modulator yokuzivikela komzimba womuntu.

Amaseli e-dendritic (ama-DC) angamaseli akhethekile aveza i-antigen anamandla kakhulu emzimbeni womuntu. Inani lama-DC emzimbeni womuntu lincane kakhulu, futhi imodeli yokungeniswa kwe-cytokine eqondiswa yi-in vitro, okungukuthi ama-DC (ama-moDC) atholakala egazini lomuntu, ivame ukusetshenziswa. Imodeli ye-DC ebangelwa yi-in vitro yabikwa okokuqala ngo-1992, okuyisistimu yokulima yendabuko yama-DC. Ngokuvamile, idinga ukutshalwa izinsuku ezingu-6-7. Amaseli omnkantsha wegundane angatshalwa nge-granulocyte macrophage colony-stimulating factor (GM-CSF) kanye ne-interleukin (IL) -4 ukuze kutholakale ama-DC angavuthwanga (iqembu le-PBS). Ama-Cytokine anezelwa njengezisusa ezivuthiwe futhi atshalwa izinsuku ezingu-1-2 ukuze kutholakale ama-DC avuthiwe. Olunye ucwaningo lubike ukuthi amaseli e-CD14+ abantu ahlanziwe akhuliswa nge-interferon – β (IFN – β) noma i-IL-4 izinsuku ezi-5, bese ekhuliswa nge-tumor necrosis factor-a (TNF-a) izinsuku ezi-2 ukuze kutholakale ama-DC anokubonakaliswa okuphezulu kwe-CD11c kanye ne-CD83, anamandla aqinile okukhuthaza ukwanda kwamaseli e-CD4+T allogeneic kanye namaseli e-CD8+T. Ama-polysaccharide amaningi avela emithonjeni yemvelo anomsebenzi omuhle kakhulu wokulawula amasosha omzimba, njenge-polysaccharides evela kumakhowe e-shiitake, amakhowe e-split gill, amakhowe e-Yunzhi, kanye ne-Poria cocos, asetshenziswe emtholampilo. Angathuthukisa ngempumelelo ukusebenza komzimba, athuthukise amasosha omzimba, futhi asebenze njengezindlela zokwelapha ezisizayo zokwelapha i-tumor. Kodwa-ke, kunemibiko embalwa yocwaningo nge-PFC njenge-immune modulator yomuntu. Ngakho-ke, lesi sihloko senza ucwaningo lokuqala ngendima kanye nezindlela ezihlobene ze-PFC ekukhuthazeni ukuvuthwa kwama-moDC, ukuze kuhlolwe amandla e-PFC njenge-immune modulator yemvelo.

Ngenxa yesilinganiso esiphansi kakhulu sama-DC ezicutshini zabantu kanye nokulondolozwa okuphezulu phakathi kwezinhlobo phakathi kwama-DC egundane nama-DC abantu, ukuze kuxazululwe ubunzima bocwaningo obubangelwa ukukhiqizwa okuphansi kwe-DC, kuye kwafundwa amamodeli okungeniswa kwe-DCs e-in vitro avela kumaseli egazi angaphandle komuntu, angathola ama-DCs anokuzivikela okuhle esikhathini esifushane. Ngakho-ke, lolu cwaningo lusebenzise indlela yendabuko yokukhuthaza ama-DC abantu e-in vitro: ukukhulisa i-rhGM CSF kanye ne-rhIL-4 e-in vitro, ukushintsha indlela yokuxhumana njalo ngosuku lwesihlanu, kanye nokuthola ama-DCs angavuthiwe ngosuku lwesihlanu; Ngosuku lwesithupha, kwanezelwa amavolumu alinganayo e-PBS, PFC, kanye ne-LPS ngokuya ngokuqoqwa kanye nokukhuliswa amahora angama-24 njengenqubo yokukhulisa yokukhuthaza ama-DCs atholakala kumaseli egazi angaphandle komuntu.

Ama-polysaccharide atholakala emikhiqizweni yemvelo anezinzuzo zokuba nobuthi obuphansi kanye nezindleko eziphansi njenge-immunostimulants. Ngemva kokuhlolwa kokuqala, iqembu lethu locwaningo lithole ukuthi i-PFC ithuthukisa kakhulu uphawu oluvuthiwe lwe-CD83 ebusweni bamaseli e-DC atholakala egazini lomuntu angaphandle kwegazi lomuntu atholakala ku-in vitro. Imiphumela ye-Flow cytometry ibonise ukuthi ukungenelela kwe-PFC ekugxilweni okungu-10 μ g/mL amahora angama-24 kuholele ekubonakalisweni okuphezulu kwe-CD83 uphawu oluvuthiwe ebusweni be-DCs, okubonisa ukuthi ama-DCs angene esimweni sokuvuthwa. Ngakho-ke, iqembu lethu locwaningo linqume uhlelo lokungeniswa nokungenelela kwe-in vitro. I-CD83 iyi-biomarker ebalulekile evuthiwe ebusweni be-DCs, kuyilapho i-CD86 isebenza njenge-molecule ebalulekile yokuvuselela ebusweni be-DCs, isebenza njengesignali yesibili yokusebenzisa amaseli e-T. Ukubonakaliswa okuthuthukisiwe kwama-biomarker amabili i-CD83 ne-CD86 kubonisa ukuthi i-PFC ikhuthaza ukuvuthwa kwama-DCs atholakala egazini lomuntu angaphandle kwegazi lomuntu, okuphakamisa ukuthi i-PFC ingakhuphula ngasikhathi sinye izinga lokukhishelwa kwama-cytokines ebusweni be-DCs. Ngakho-ke, lolu cwaningo luhlole amazinga ama-cytokines IL-6, TNF-a, kanye ne-IL-10 akhishwa yi-DCs kusetshenziswa i-ELISA. I-IL-10 ihlobene kakhulu nokubekezelelana kwamasosha omzimba kwama-DCs, kanti ama-DCs anokubekezelelana kwamasosha omzimba avame ukusetshenziswa ekwelashweni kwesimila, enikeza imibono yokwelapha engaba khona yokubekezelelana kwamasosha omzimba ekufakweni kwezitho; Umndeni we-1L-6 udlala indima ebalulekile ekuvikeleni kwangaphakathi nangokwezimo, i-hematopoiesis, kanye nemiphumela yokulwa nokuvuvukala; Kunezifundo ezibonisa ukuthi i-IL-6 kanye ne-TGF β zihlanganyela ndawonye ekuhlukaniseni amaseli e-Th17; Lapho umzimba uhlaselwa yigciwane, i-TNF-a ekhiqizwa yi-DCs ekuphenduleni ukusebenza kwegciwane isebenza njengesici sokuvuthwa kwe-autocrine ukukhuthaza ukuvuthwa kwe-DC. Ukuvimba i-TNF-a kuzobeka ama-DCs esigabeni sokungavuthwa, kuwavimbele ekusebenziseni ngokugcwele umsebenzi wawo wokwethula i-antigen. Idatha ye-ELISA kulolu cwaningo ikhombisile ukuthi izinga lokukhishelwa kwe-IL-10 eqenjini le-PFC landa kakhulu uma kuqhathaniswa namanye amaqembu amabili, okubonisa ukuthi i-PFC ithuthukisa ukubekezelelana kwamasosha omzimba kwama-DCs; Amazinga okwanda kokukhishwa kwe-IL-6 kanye ne-TNF-a asikisela ukuthi i-PFC ingaba nomthelela wokuthuthukisa i-DC ukukhuthaza ukuhlukaniswa kwamaseli e-T.