Polysaccharide-ka laga helo Chlorella (PFC), oo ah polysaccharide dabiici ah, ayaa soo jiidatay dareenka badan aqoonyahannada sannadihii ugu dambeeyay sababtoo ah faa'iidooyinkeeda sunta yar, waxyeellooyinka yar, iyo saamaynta ballaaran. Shaqooyinkeeda hoos u dhigista dufanka dhiigga, ka hortagga burooyinka, ka hortagga bararka, ka hortagga Parkinson, ka hortagga gabowga, iwm. waxaa si horudhac ah loo xaqiijiyay tijaabooyinka in vitro iyo in vivo. Si kastaba ha ahaatee, weli waxaa jira farqi ku jira cilmi-baarista ku saabsan PFC oo ah habeeyaha difaaca jirka ee aadanaha.

Unugyada Dendritic (DCs) waa unugyada ugu awoodda badan ee soo bandhiga antigen-ka ee jirka aadanaha. Tirada DC-yada ku jira jirka aadanaha aad bay u yar yihiin, waxaana si caadi ah loo isticmaalaa qaabka soo saarista cytokine ee in vitro, oo ah DC-yada unugyada mononuclear-ka ee laga helo dhiigga aadanaha (moDCs),. Qaabka DC ee in vitro-ga lagu sameeyay ayaa markii ugu horreysay la soo sheegay 1992, kaas oo ah nidaamka dhaqanka dhaqameed ee DC-yada. Guud ahaan, waxay u baahan tahay beeris 6-7 maalmood. Unugyada lafta jiirka waxaa lagu beeri karaa iyadoo la adeegsanayo granulocyte macrophage colony-stimulating factor (GM-CSF) iyo interleukin (IL) -4 si loo helo DC-yada aan qaan gaarin (kooxda PBS). Cytokines-ka waxaa lagu daraa kicinta bisil waxaana la beeraa 1-2 maalmood si loo helo DC-yada bisil. Daraasad kale ayaa sheegtay in unugyada CD14+ ee aadanaha ee la safeeyey lagu beeray interferon - β (IFN - β) ama IL-4 muddo 5 maalmood ah, ka dibna lagu beeray burooyinka necrosis factor-a (TNF-a) muddo 2 maalmood ah si loo helo DCs leh muujinta sare ee CD11c iyo CD83, kuwaas oo leh awood xooggan oo lagu horumarin karo faafitaanka unugyada allogeneic CD4+T iyo unugyada CD8+T. Polysaccharides badan oo ka yimid ilo dabiici ah ayaa leh firfircooni difaac oo heer sare ah, sida polysaccharides laga helo boqoshaada shiitake, boqoshaada gill-ka kala qaybsan, boqoshaada Yunzhi, iyo Poria cocos, kuwaas oo lagu dabaqay dhaqanka caafimaadka. Waxay si wax ku ool ah u hagaajin karaan shaqada difaaca jirka, waxay kor u qaadi karaan difaaca jirka, waxayna u adeegi karaan daaweyn kaaliye ah oo loogu talagalay daaweynta ka hortagga burooyinka. Si kastaba ha ahaatee, waxaa jira warbixino cilmi baaris oo yar oo ku saabsan PFC oo ah modulator difaaca jirka aadanaha. Sidaa darteed, maqaalkani wuxuu sameeyaa cilmi-baaris hordhac ah oo ku saabsan doorka iyo hababka la xiriira ee PFC ee kor u qaadista bislaanshaha moDCs, si loo qiimeeyo awoodda PFC oo ah modulator difaac dabiici ah.

Sababtoo ah saamiga aadka u hooseeya ee DC-yada unugyada aadanaha iyo ilaalinta noocyada kala duwan ee u dhexeeya DC-yada jiirka iyo DC-yada aadanaha, si loo xalliyo dhibaatooyinka cilmi-baarista ee ay keento wax soo saarka DC oo hooseeya, moodooyinka soo-saarista ee in vitro-ga ee DC-yada laga soo qaatay unugyada mononuclear-ka dhiigga ee aadanaha ayaa la bartay, kuwaas oo heli kara DC-yo leh difaac wanaagsan muddo gaaban gudaheed. Sidaa darteed, daraasaddani waxay isticmaashay habka dhaqameed ee lagu kicinayo DC-yada aadanaha ee in vitro-ga: isku-darka rhGM CSF iyo rhIL-4 in vitro, beddelidda dhexdhexaadka maalin kasta, iyo helitaanka DC-yada aan qaan-gaarin maalinta 5aad; Maalintii 6aad, mugga isku midka ah ee PBS, PFC, iyo LPS ayaa lagu daray iyadoo loo eegayo kooxaynta waxaana la beeray 24 saacadood iyagoo ah hab-maamuuska dhaqanka ee lagu kicinayo DC-yada laga soo qaatay unugyada mononuclear-ka dhiigga ee aadanaha.

Polysaccharides-ka laga soo saaro alaabada dabiiciga ah waxay leeyihiin faa'iidooyinka sunta yar iyo kharash yar oo ah kicinta difaaca jirka. Ka dib tijaabooyin hordhac ah, kooxdeenna cilmi-baarista waxay ogaatay in PFC ay si weyn u xoojiso calaamadda qaan-gaarka ah CD83 dusha sare ee unugyada DC-ga ee laga soo saaro unugyada ... Sidaa darteed, daraasaddani waxay qiimaysay heerarka cytokines-ka IL-6, TNF-a, iyo IL-10 oo ay soo saaraan DC-yada iyagoo adeegsanaya ELISA. IL-10 waxay si dhow ula xiriirtaa dulqaadka difaaca ee DC-yada, DC-yada leh dulqaadka difaaca jirka ayaa si caadi ah loogu isticmaalaa daaweynta burooyinka, iyagoo bixinaya fikrado daaweyn oo suurtagal ah oo loogu talagalay dulqaadka difaaca jirka ee ku-tallaalidda xubnaha; Qoyska 1L-6 waxay door muhiim ah ka ciyaaraan difaac dabiici ah iyo mid la qabsan kara, hematopoiesis, iyo saameynta ka hortagga bararka; Waxaa jira daraasado muujinaya in IL-6 iyo TGF β ay si wadajir ah uga qayb qaataan kala soocidda unugyada Th17; Marka jirka uu ku soo duulo fayras, TNF-a oo ay soo saaraan DC-yada iyagoo ka jawaabaya firfircoonida fayrasku waxay u dhaqmaan sidii arrin bislaanshaha autocrine si kor loogu qaado bislaanshaha DC. Xannibaadda TNF-a waxay DC-yada gelin doontaa marxalad aan qaan gaarin, taasoo ka hortagaysa inay si buuxda u fuliyaan shaqadooda soo bandhigidda antigen-ka. Xogta ELISA ee daraasaddan waxay muujisay in heerka dheecaanka IL-10 ee kooxda PFC uu si weyn u kordhay marka la barbar dhigo labada kooxood ee kale, taasoo muujinaysa in PFC ay kor u qaaddo dulqaadka difaaca jirka ee DC-yada; Heerarka sii kordhaya ee dheecaanka IL-6 iyo TNF-a waxay soo jeedinayaan in PFC ay yeelan karto saameynta kor u qaadista DC si kor loogu qaado kala soocidda unugyada T.