Kwimimandla eyahlukeneyo yophando kunye nokusetyenziswa kwe-microalgae, itekhnoloji yokugcina iiseli ze-microalgae ixesha elide ibalulekile. Iindlela zemveli zokugcina ii-microalgae zijongene nemingeni emininzi, kubandakanya ukuncipha kozinzo lwezakhi zofuzo, ukunyuka kweendleko, kunye nokwanda kweengozi zongcoliseko. Ukujongana nale micimbi, i-protoga iphuhlise indlela yokugcina i-vitrification cryopreservation efanelekileyo kwiintlobo ngeentlobo ze-microalgae. Ukwenziwa kwesisombululo se-cryopreservation kubalulekile ekugcineni amandla kunye nozinzo lwezakhi zofuzo zeeseli ze-microalgae.

Okwangoku, nangona izicelo eziphumeleleyo zenziwe kwiChlamydomonas reinhardtii, umahluko kwisakhiwo se-physiological kunye neseli phakathi kweentlobo ezahlukeneyo ze-microalgae kuthetha ukuba i-microalgae nganye inokufuna iindlela ezithile zokukhusela i-cryoprotectant. Xa kuthelekiswa nezisombululo zokukhusela i-cryopreservation ezisetyenziswa kwezinye iindlela zokukhusela i-microbial kunye ne-alwany cell cell, isisombululo sokukhusela i-cryopreservation se-microalgae kufuneka siqwalasele ulwakhiwo lodonga lweseli, ukumelana neqhwa, kunye neempendulo ezithile ezinobuthi ze-protectorants kwiiseli ze-microalgae zeentlobo ezahlukeneyo ze-algal.

Itekhnoloji yokugcinwa kwe-vitrification cryopreservation ye-microalgae isebenzisa izisombululo zokugcinwa kwe-cryopreservation ezenzelwe ngokukodwa ukugcina iiseli kumaqondo obushushu aphantsi kakhulu, njenge-nitrogen engamanzi okanye -80 ° C, emva kwenkqubo yokupholisa ecwangcisiweyo. Iikristale zomkhenkce zihlala zenzeka ngaphakathi kwiiseli ngexesha lokupholisa, zibangela umonakalo kwisakhiwo seseli kunye nokulahleka komsebenzi weseli, nto leyo ekhokelela ekufeni kweseli. Ukuze kuphuhliswe izisombululo zokugcinwa kwe-microalgae cryopreservation, i-protoga yenze uphando olunzulu malunga neempawu zeseli ze-microalgae, kubandakanya iimpendulo zazo kwizithinteli ezahlukeneyo kunye nendlela yokunciphisa ngempumelelo umonakalo obangelwa kukuqandisa kunye noxinzelelo lwe-osmotic. Oku kubandakanya uhlengahlengiso oluqhubekayo kuhlobo, uxinaniso, ulandelelwano lokongeza, iinkqubo zangaphambi kokupholisa, kunye neenkqubo zokubuyisela izinto ezikhuselayo kwisisombululo sokugcinwa kwe-cryopreservation, okukhokelela ekuphuhlisweni kwesisombululo sokugcinwa kwe-microalgae cryopreservation esibanzi esibizwa ngokuba yiFroznthrive ™ kunye netekhnoloji yokugcinwa kwe-vitrification exhasayo.