Munzvimbo dzakasiyana-siyana dzekutsvaga nekushandisa ma microalgae, tekinoroji yekuchengetedza masero e microalgae kwenguva refu yakakosha. Nzira dzechinyakare dzekuchengetedza ma microalgae dzinosangana nematambudziko akawanda, kusanganisira kudzikira kwekugadzikana kwemajini, kuwedzera kwemitengo, uye kuwedzera kwenjodzi dzekusvibiswa. Kuti vagadzirise matambudziko aya, protoga yakagadzira nzira yekuchengetedza vitrification yakakodzera ma microalgae akasiyana-siyana. Kugadzirwa kwe cryopreservation solution kwakakosha pakuchengetedza simba uye kugadzikana kwemajini emasero e microalgae.

Parizvino, kunyangwe kushandiswa kwakabudirira kwakaitwa paChlamydomonas reinhardtii, musiyano wemuviri uye wemasero pakati pemarudzi akasiyana e microalgae zvinoreva kuti microalgae yega yega inogona kuda maforoma chaiwo ekudzivirira cryoprotectant. Kana ichienzaniswa nemhinduro dzekuchengetedza cryopreservation dzinoshandiswa mune dzimwe nzira dzekuchengetedza cryopreservation dzema microbial nemhuka, mhinduro yekudzivirira cryopreservation ye microalgae inofanirwa kufunga nezvechimiro chemadziro esero, kuramba chando, uye maitiro chaiwo echepfu ekudzivirira kumasero e microalgae emarudzi akasiyana ealgal.

Tekinoroji yekudzivirira kupisa kwemasero mu microalgae inoshandisa mhinduro dzakagadzirirwa kuchengetedza masero patembiricha yakaderera zvikuru, senge liquid nitrogen kana -80 ° C, mushure mekutonhodza kwakarongwa. Makristaro echando anowanzo kuumbwa mukati memasero panguva yekutonhodza, zvichikonzera kukuvara kwechimiro chemasero uye kurasikirwa nebasa remasero, zvichikonzera kufa kwemasero. Kuti vagadzire mhinduro dzekuchengetedza kupisa kwemasero mu microalgae, protoga yakaita tsvakiridzo yakadzama pamusoro pehunhu hwemasero e microalgae, kusanganisira maitiro avo kune akasiyana anodzivirira uye maitiro ekuderedza kukuvara kunokonzerwa nechando ne osmotic pressure. Izvi zvinosanganisira kugadzirisa kunoramba kuripo kune mhando, huwandu, kuwedzera, pre cooling, uye kudzoreredza maitiro ekudzivirira mu cryopreservation solution, zvichikonzera kugadzirwa kwe broad-spectrum microalgae cryopreservation solution inonzi Froznthrive ™ Uye tekinoroji inotsigira vitrification freezing.