Mafapheng a fapaneng a lipatlisiso le ts'ebeliso ea makhopho a manyane, theknoloji ea ho boloka lisele tsa makhopho a manyane nako e telele e bohlokoa haholo. Mekhoa ea setso ea ho boloka makhopho a manyane e tobana le liphephetso tse ngata, ho kenyeletsoa ho fokotseha ha botsitso ba liphatsa tsa lefutso, litšenyehelo tse eketsehileng, le likotsi tse eketsehileng tsa tšilafalo. Ho rarolla mathata ana, protoga e hlahisitse mokhoa oa ho boloka makhopho a manyane a loketseng makhopho a manyane a fapaneng. Ho etsoa ha tharollo ea ho boloka makhopho ho bohlokoa bakeng sa ho boloka matla le botsitso ba liphatsa tsa lefutso ba lisele tsa makhopho a manyane.

Hona jwale, le hoja ditshebediso tse atlehileng di se di entswe ho Chlamydomonas reinhardtii, dipharologanyo tsa sebopeho sa mmele le sa sele pakeng tsa mefuta e fapaneng ya di-microalgae di bolela hore microalgae ka nngwe e ka hloka diforomo tse ikgethang tsa cryoprotectant. Ha e bapiswa le ditharollo tsa cryopreservation tse sebediswang mekgweng e meng ya ho boloka disele tsa dikokwana-hloko le tsa diphoofolo, tharollo ya cryopreservation bakeng sa microalgae e hloka ho nahana ka sebopeho sa lebota la sele, ho hanyetsa serame, le dikarabelo tse itseng tse chefo tsa di-protector ho disele tsa microalgae tsa mefuta e fapaneng ya di-algae.

Theknoloji ea ho boloka matla a khoheli ea microalgae e sebelisa litharollo tsa ho boloka matla a khoheli tse entsoeng ka mokhoa o khethehileng ho boloka lisele mochesong o tlase haholo, joalo ka naetrojene e metsi kapa -80 ° C, kamora ts'ebetso ea ho pholisa e hlophisitsoeng. Likristale tsa leqhoa hangata li thehoa ka hare ho lisele nakong ea ho pholisa, li baka tšenyo sebopehong sa sele le tahlehelo ea ts'ebetso ea sele, e leng se lebisang lefung la sele. E le ho nts'etsapele litharollo tsa ho boloka matla a khoheli tsa microalgae, protoga e entse lipatlisiso tse tebileng mabapi le litšobotsi tsa sele tsa microalgae, ho kenyeletsoa le karabelo ea tsona ho li-protector tse fapaneng le mokhoa oa ho fokotsa tšenyo e bakoang ke ho hoama le khatello ea osmotic ka katleho. Sena se kenyelletsa liphetoho tse tsoelang pele ho mofuta, mahloriso, tatellano ea ho eketsa, mekhoa ea pele ho pholisa le ea ho hlaphoheloa ea li-agent tse sireletsang tharollong ea ho boloka matla a khoheli, e leng se fellang ka nts'etsopele ea tharollo ea ho boloka matla a khoheli ea microalgae e pharaletseng e bitsoang Froznthrive ™ Le theknoloji e tšehetsang ea ho hoama ea vitrification.