Mu nzego zitandukanye z’ubushakashatsi n’ikoreshwa ry’uturemangingo duto, ikoranabuhanga ryo kubungabunga uturemangingo duto tw’uturemangingo mu gihe kirekire ni ingenzi cyane. Uburyo gakondo bwo kubungabunga uturemangingo duto tw’uturemangingo buhura n’imbogamizi nyinshi, harimo kugabanuka kw’ubudahangarwa bw’uturemangingo, kwiyongera kw’ibiciro, no kwiyongera kw’ibyago byo kwandura. Kugira ngo ibyo bibazo bikemuke, protoga yashyizeho uburyo bwo kubika amazi mu buryo bwa vitrification bukwiriye uturemangingo duto duto tw’uturemangingo. Gutegura umuti wo kubika amazi mu buryo bwa cryopreservation ni ingenzi cyane mu kubungabunga ubuzima n’ubudahangarwa bw’uturemangingo duto tw’uturemangingo.

Muri iki gihe, nubwo habayeho gukoresha neza Chlamydomonas reinhardtii, itandukaniro riri hagati y’imiterere y’ibinyabuzima n’imiterere y’uturemangingo hagati y’ubwoko butandukanye bw’uturemangingo bivuze ko buri turemangingo dushobora gukenera uburyo bwihariye bwo kurinda udukoko. Ugereranije n’uburyo bwo kurinda udukoko tubukoresha mu bundi buryo bwo kurinda udukoko tubukoresha mu tundi dukoko tubukoresha mu kurinda udukoko tubukoresha mu turemangingo ...

Ikoranabuhanga ryo kubika amazi mu buryo bwa vitrification cryopreservation rya microalgae rikoresha uburyo bwihariye bwo kubika amazi mu buryo bwa cryopreservation kugira ngo bibike uturemangingo ku bushyuhe buri hasi cyane, nka azote y'amazi cyangwa -80 ° C, nyuma yo gukonjesha byateguwe. Uturemangingo tw'urubura akenshi tuba mu turemangingo mu gihe cyo gukonjesha, bigatera kwangirika kw'imiterere y'uturemangingo no gutakaza imikorere y'uturemangingo, bigatera urupfu rw'uturemangingo. Kugira ngo hatezweho uburyo bwo kubika amazi mu buryo bwa microalgae, protoga yakoze ubushakashatsi bwimbitse ku miterere y'uturemangingo twa microalgae, harimo n'uko bikora ku bintu bitandukanye birinda ndetse n'uburyo bwo kugabanya neza ibyangiritse biterwa no gukonjesha n'umuvuduko wa osmotic. Ibi birimo guhindura buri gihe ubwoko, ubwinshi, uburyo bwo kongeramo, uburyo bwo gukonjesha mbere yo gukonjesha, no kugarura ibintu birinda mu buryo bwa cryopreservation, bigatuma habaho uburyo bwa microalgae cryopreservation bukoreshwa mu buryo bwagutse bwitwa Froznthrive ™ hamwe n'ikoranabuhanga rifasha mu gukonjesha amazi mu buryo bwa vitrification.