A fannoni daban-daban na bincike da amfani da ƙananan algae, fasahar adana ƙwayoyin ƙananan algae na dogon lokaci tana da matuƙar muhimmanci. Hanyoyin adana ƙananan algae na gargajiya suna fuskantar ƙalubale da yawa, ciki har da raguwar kwanciyar hankali na kwayoyin halitta, ƙaruwar farashi, da kuma ƙaruwar haɗarin gurɓatawa. Don magance waɗannan matsalolin, protoga ta ƙirƙiro wata dabarar adana ƙwayoyin ƙwayoyin halitta da ta dace da nau'ikan ƙananan algae. Tsarin maganin adana ƙwayoyin halitta yana da matuƙar muhimmanci don kiyaye kuzari da kwanciyar hankali na ƙwayoyin ƙananan algae.

A halin yanzu, kodayake an yi nasarar amfani da Chlamydomonas reinhardtii, bambance-bambancen tsarin jiki da na tantanin halitta tsakanin nau'ikan microalgae daban-daban yana nufin cewa kowace microalgae na iya buƙatar takamaiman tsarin cryoprotectant. Idan aka kwatanta da maganin cryopreservation da ake amfani da shi a wasu dabarun cryopreservation na ƙwayoyin cuta da na dabbobi, maganin cryopreservation na ƙwayoyin microalgae yana buƙatar la'akari da tsarin bangon tantanin halitta, juriya ga sanyi, da takamaiman halayen guba na masu kariya ga ƙwayoyin microalgae na nau'ikan algae daban-daban.

Fasahar cryopreservation ta microalgae tana amfani da mafita na cryopreservation da aka tsara musamman don adana ƙwayoyin halitta a yanayin zafi mai ƙanƙanta, kamar ruwa nitrogen ko -80 ° C, bayan an tsara tsarin sanyaya. Kwalliyar kankara yawanci tana samuwa a cikin ƙwayoyin halitta yayin sanyaya, tana haifar da lalacewar tsarin ƙwayoyin halitta da asarar aikin ƙwayoyin halitta, wanda ke haifar da mutuwar ƙwayoyin halitta. Domin haɓaka mafita na cryopreservation na microalgae, protoga sun gudanar da bincike mai zurfi kan halayen ƙwayoyin halitta na microalgae, gami da halayensu ga masu kariya daban-daban da kuma yadda za a rage lalacewar da daskarewa da matsin lamba na osmotic ke haifarwa yadda ya kamata. Wannan ya haɗa da ci gaba da daidaitawa ga nau'in, maida hankali, jerin ƙari, kafin sanyaya, da hanyoyin dawo da sinadarai masu kariya a cikin maganin cryopreservation, wanda ke haifar da haɓaka mafita mai faɗi na microalgae cryopreservation da ake kira Froznthrive ™ Da fasahar daskarewa ta vitrification mai tallafawa.