M'magawo osiyanasiyana ofufuza ndi kugwiritsa ntchito ma microalgae, ukadaulo wosunga maselo a microalgae kwa nthawi yayitali ndi wofunikira kwambiri. Njira zosungira ma microalgae zachikhalidwe zimakumana ndi mavuto ambiri, kuphatikizapo kuchepa kwa kukhazikika kwa majini, kukwera kwa ndalama, komanso kuwonjezeka kwa zoopsa zoipitsa. Pofuna kuthana ndi mavutowa, protoga yapanga njira yosungira ma vitrification cryopreservation yoyenera ma microalgae osiyanasiyana. Kupanga njira yosungira ma cryopreservation ndikofunikira kwambiri kuti maselo a microalgae akhale ndi mphamvu komanso kukhazikika kwa majini.

Pakadali pano, ngakhale kuti ntchito zabwino zagwiritsidwa ntchito pa Chlamydomonas reinhardtii, kusiyana kwa kapangidwe ka thupi ndi maselo pakati pa mitundu yosiyanasiyana ya ma microalgae kumatanthauza kuti microalgae iliyonse ingafunike njira zina zotetezera ma cryoprotectant. Poyerekeza ndi njira zotetezera ma cryopreservation zomwe zimagwiritsidwa ntchito mu njira zina zotetezera ma microbial ndi ma cell a nyama, njira yotetezera ma cryopreservation ya microalgae iyenera kuganizira kapangidwe ka khoma la selo, kukana chisanu, ndi momwe zinthu zotetezera zimakhudzira ma microalgae amitundu yosiyanasiyana ya algae.

Ukadaulo wa vitrification cryopreservation wa microalgae umagwiritsa ntchito njira zapadera zosungira cryopreservation kuti usunge maselo pa kutentha kochepa kwambiri, monga nayitrogeni yamadzimadzi kapena -80 ° C, pambuyo pa njira yozizira yokonzedweratu. Makristalo a ayezi nthawi zambiri amapangika mkati mwa maselo panthawi yozizira, zomwe zimapangitsa kuti kapangidwe ka maselo kawonongeke komanso kutayika kwa ntchito ya maselo, zomwe zimapangitsa kuti maselo afe. ​​Pofuna kupanga njira zosungira cryopreservation za microalgae, protoga idachita kafukufuku wozama pa makhalidwe a maselo a microalgae, kuphatikizapo momwe amachitira ndi zoteteza zosiyanasiyana komanso momwe angachepetsere kuwonongeka komwe kumachitika chifukwa cha kuzizira ndi kuthamanga kwa osmotic. Izi zikuphatikizapo kusintha kosalekeza kwa mtundu, kuchuluka, kutsatana, kuzizira koyambirira, ndi njira zobwezeretsa zoteteza mu yankho losungira cryopreservation, zomwe zimapangitsa kuti pakhale njira yosungira cryopreservation ya microalgae yotchedwa Froznthrive ™. Ndi ukadaulo wothandizira woziziritsa vitrification.