Hauv ntau qhov chaw ntawm kev tshawb fawb thiab daim ntawv thov microalgae, cov thev naus laus zis ntawm kev khaws cia ntev ntawm cov hlwb microalgae yog qhov tseem ceeb heev. Cov txheej txheem khaws cia microalgae ib txwm ntsib ntau yam teeb meem, suav nrog kev ruaj khov ntawm cov noob caj noob ces txo qis, cov nqi nce ntxiv, thiab kev pheej hmoo ntawm kev ua qias tuaj ntxiv. Txhawm rau daws cov teeb meem no, protoga tau tsim cov txheej txheem vitrification cryopreservation uas haum rau ntau yam microalgae. Kev tsim cov tshuaj cryopreservation yog qhov tseem ceeb rau kev tswj hwm lub zog thiab kev ruaj khov ntawm cov hlwb microalgae.

Tam sim no, txawm hais tias muaj kev siv tau zoo rau Chlamydomonas reinhardtii, qhov sib txawv ntawm lub cev thiab cov qauv ntawm cov kab mob me me sib txawv txhais tau tias txhua tus kab mob me me yuav xav tau cov tshuaj cryoprotectant tshwj xeeb. Piv nrog rau cov tshuaj cryopreservation siv rau hauv lwm cov txheej txheem cryopreservation ntawm cov kab mob me me thiab tsiaj, cov tshuaj cryopreservation rau microalgae yuav tsum xav txog cov qauv ntawm phab ntsa ntawm tes, kev tiv thaiv te, thiab cov tshuaj lom tshwj xeeb ntawm cov tshuaj tiv thaiv rau cov kab mob me me ntawm cov kab mob algae sib txawv.

Cov thev naus laus zis vitrification cryopreservation ntawm microalgae siv cov kev daws teeb meem cryopreservation tsim tshwj xeeb los khaws cov cell ntawm qhov kub qis heev, xws li kua nitrogen lossis -80 ° C, tom qab cov txheej txheem txias programmed. Cov dej khov crystals feem ntau tsim sab hauv cov cell thaum lub sijhawm txias, ua rau puas tsuaj rau cov qauv cell thiab poob ntawm kev ua haujlwm ntawm cell, ua rau cell tuag. Txhawm rau tsim cov kev daws teeb meem cryopreservation microalgae, protoga tau ua kev tshawb fawb tob tob txog cov yam ntxwv ntawm cell ntawm microalgae, suav nrog lawv cov tshuaj tiv thaiv rau ntau yam kev tiv thaiv thiab yuav ua li cas kom txo qhov kev puas tsuaj los ntawm kev khov thiab osmotic siab. Qhov no suav nrog kev hloov kho tas mus li rau hom, concentration, ntxiv cov kab ke, ua ntej txias, thiab cov txheej txheem rov qab ntawm cov neeg sawv cev tiv thaiv hauv cov tshuaj cryopreservation, ua rau muaj kev tsim cov tshuaj cryopreservation microalgae dav dav hu ua Froznthrive ™ Thiab cov thev naus laus zis khov kho vitrification txhawb nqa.