I roto i ngā momo mara rangahau me te whakamahinga o ngā moroiti, he mea nui te hangarau tiaki roa o ngā pūtau moroiti. He maha ngā wero e pā ana ki ngā tikanga tiaki moroiti tuku iho, tae atu ki te hekenga o te pumau ira, te pikinga o ngā utu, me te pikinga o ngā mōrearea parahanga. Hei whakatika i ēnei take, kua whakawhanakehia e Protoga tētahi tikanga tiaki-kirikiri whakakīkī e tika ana mō ngā momo moroiti. He mea nui te hanganga o te otinga tiaki-kirikiri hei pupuri i te ora me te pumau ira o ngā pūtau moroiti.

I tēnei wā, ahakoa kua angitu ngā tono ki te Chlamydomonas reinhardtii, ko ngā rerekētanga hanganga ā-tinana me te hanganga pūtau i waenga i ngā momo moroiti rerekē e tohu ana ka hiahiatia pea he whakatakotoranga tiaki-whakamarumaru motuhake mō ia moroiti. Ki te whakatauritea ki ngā otinga tiaki-whakamarumaru e whakamahia ana i roto i ētahi atu tikanga tiaki-whakamarumaru pūtau moroiti me ngā pūtau kararehe, me whai whakaaro te otinga tiaki-whakamarumaru mō ngā moroiti ki te hanganga o te pakitara pūtau, te ātete huka, me ngā tauhohenga paitini motuhake o ngā tiaki ki ngā pūtau moroiti o ngā momo moroiti rerekē.

Ko te hangarau tiaki-whakapūmau o ngā moroiti ka whakamahi i ngā otinga tiaki-whakapūmau motuhake hei penapena i ngā pūtau i ngā pāmahana tino iti, pērā i te hauota wai, i te -80°C rānei, i muri i te tukanga whakamatao kua whakaritea. Ko ngā tioata hukapapa ka puta i roto i ngā pūtau i te wā whakamatao, ka pakaru te hanganga pūtau me te ngaronga o te mahi a te pūtau, ka mate te pūtau. Hei whakawhanake i ngā otinga tiaki-whakapūmau moroiti, i whakahaerehia e Protoga he rangahau hōhonu mō ngā āhuatanga pūtau o ngā moroiti, tae atu ki ā rātou tauhohenga ki ngā momo tiaki me te pehea e tino whai hua ai te whakaiti i te kino i puta mai i te tio me te pēhanga osmotic. Kei roto i tēnei ko ngā whakarerekētanga tonu ki te momo, te kukū, te raupapa tāpiri, te whakamatao i mua, me ngā tukanga whakaora o ngā kaihoko tiaki i roto i te otinga tiaki-whakapūmau, ka hua ake te whanaketanga o tētahi otinga tiaki-whakapūmau moroiti whānui e kiia nei ko Froznthrive ™ Me te hangarau tio tautoko i te whakatikatika.