Emikhakheni eyahlukene yocwaningo kanye nokusetshenziswa kwe-microalgae, ubuchwepheshe bokulondolozwa kwesikhathi eside kwamaseli e-microalgae bubalulekile. Izindlela zokulondolozwa kwe-microalgae zendabuko zibhekene nezinselele eziningi, okuhlanganisa ukwehla kokuqina kwezakhi zofuzo, ukwanda kwezindleko, kanye nokwanda kwezingozi zokungcola. Ukuze kuxazululwe lezi zinkinga, i-protoga ithuthukise indlela yokuvikela i-vitrification cryopreservation efanelekela ama-microalgae ahlukahlukene. Ukwakhiwa kwesisombululo se-cryopreservation kubalulekile ekugcineni amandla kanye nokuqina kwezakhi zofuzo zamaseli e-microalgae.

Njengamanje, nakuba ukusetshenziswa okuphumelelayo kwenziwe ku-Chlamydomonas reinhardtii, umehluko wesakhiwo somzimba kanye nesamangqamuzana phakathi kwezinhlobo ezahlukene ze-microalgae usho ukuthi i-microalgae ngayinye ingadinga ukwakheka kwe-cryoprotectant ethile. Uma kuqhathaniswa nezixazululo ze-cryopreservation ezisetshenziswa kwezinye izindlela ze-microbial kanye ne-animal cell cryopreservation, isixazululo se-cryopreservation se-microalgae sidinga ukucabangela isakhiwo sodonga lweseli, ukumelana neqhwa, kanye nokusabela okunobuthi okuthile kwezivikeli kumaseli e-microalgae ezinhlobo ezahlukene ze-algal.

Ubuchwepheshe bokugcina i-vitrification cryopreservation be-microalgae busebenzisa izixazululo zokulondoloza i-cryopreservation eziklanywe ngokukhethekile ukugcina amaseli emazingeni okushisa aphansi kakhulu, njenge-nitrogen ewuketshezi noma -80 ° C, ngemva kwenqubo yokupholisa ehleliwe. Amakristalu eqhwa avame ukwakheka ngaphakathi kwamaseli ngesikhathi sokupholisa, okubangela umonakalo esakhiweni seseli kanye nokulahlekelwa umsebenzi weseli, okuholela ekufeni kwamaseli. Ukuze kuthuthukiswe izixazululo zokulondoloza i-microalgae cryopreservation, i-protoga yenze ucwaningo olujulile ngezici zamaseli ze-microalgae, okuhlanganisa ukusabela kwazo ezivikelweni ezahlukene nokuthi inganciphisa kanjani ngempumelelo umonakalo obangelwa ukubanda kanye nokucindezela kwe-osmotic. Lokhu kufaka phakathi ukulungiswa okuqhubekayo kohlobo, ukuhlushwa, ukulandelana kokwengeza, izinqubo zangaphambi kokupholisa, kanye nokubuyiselwa kwezinto ezivikelayo esixazululweni sokulondoloza i-cryopreservation, okuholela ekuthuthukisweni kwesisombululo sokulondoloza i-microalgae cryopreservation esibanzi esibizwa ngokuthi i-Froznthrive ™ kanye nobuchwepheshe bokuqanda i-vitrification obusekelayo.